American drama life begins with Dr. House

157 Four methods of making vaccines (5000 words~)

Seeing the familiar torture kit, Amherst's eyes jumped wildly.

The injury on his finger had just scabbed over.

The feeling of having my nails pulled out makes my heart tremble when I think about it now.

He looked at the unsmiling three people in front of him who had put on leather aprons and gloves and began to feel scared from the bottom of his heart.

He is not afraid of sacrificing his life for his ideals, but that doesn't mean he is willing to live a life worse than death.

So Amherst said before Reese's hand touched the torture bag:

"Wait, I really didn't lie just now.

I replaced the smallpox virus gene in the polio virus.

That's why the original poliovirus vaccine lost its effectiveness.

Essentially this is a new virus.

If you want to develop a vaccine, there are only four ways, namely inactivated vaccine, genetically engineered recombinant subunit vaccine, vector vaccine and nucleic acid vaccine.

Inactivated vaccines require the pathogens to be inactivated before they are made into vaccines.

But the pathogen of this new virus cannot be killed by current methods, which means that this road has been cut off.

Then there is the genetically engineered recombinant subunit vaccine, which mainly involves finding the part of the gene on the poliovirus that is responsible for 'producing' the protein.

Then use microorganisms as a safe 'petri dish' to produce the S protein we need, and finally make a vaccine.

but……"

At this point Amherst smiled bitterly:

"I had thought of this possibility when I was developing the virus, so I erased the part of the gene fragment related to the S protein, so this method didn't work either.

As for the other two methods that may be effective..."

Amherst suddenly fell silent and turned to look at Reese in front of him.

"I'm a little thirsty. Can you get me some water?"

Seeing Rise's unmoved look, Amherst smiled and continued:

"Are you worried that I'm cheating? You see, my hands and feet are tightly bound by you, and I can't do anything I want to do.

My throat is just a little thirsty, after all, I haven't eaten for a long time.

My stomach was empty, which made my mind a little empty too.

I can’t seem to remember the remaining two possible methods.”

Seeing that Amherst was actually taking advantage of everyone's weaknesses to threaten everyone, the taciturn Michael sneered, picked up the vise Duwei had used and walked towards Amherst.

Amherst's heart tightened, and when he was about to beg for mercy, Reese stopped McKell and said:

"If the remaining two methods really work, we still need him to research a vaccine for us.

There is no benefit in crippling him now, I guarantee that if he dares not to say anything, I will be the first to do it."

After McKell looked straight at Rise for a while, he threw the vise back on the table:

"In that case, I'll leave this place to you."

Seeing that McKell left here directly, Reese turned his attention to Frank, who was always more talkative.

Frank shrugged:

"I don't care anymore, no matter what, he will still speak in the end.

In that case, let me go get him something to eat and drink."

Soon, Amherst, who had not eaten for a long time, received a supplement. He licked his chapped lips with some satisfaction and smiled:

"That's right. Preferential treatment of prisoners of war is an internationally recognized law. Besides..."

Seeing that Amherst still wanted to make some demands, Reese frowned and said:

"Okay, if you don't want to suffer, just hurry up!"

Seeing Reese's determination, Amherst shrank back and stopped pushing further.

"The third method is vector vaccine. Simply put, the gene that can produce S protein is integrated into adenovirus or attenuated influenza virus, and then the human body is infected with these viruses.

Then the human body can naturally synthesize S protein.

And because adenovirus or attenuated influenza virus is just a vector, they will not actually make the human body sick.

It's like your vehicle originally carried a load of bombs, but now it carries a life-saving medical kit..."

After hearing this, Risse frowned more and more.

The new method Amherst said cannot bypass the gene on the poliovirus that produces the S protein.

But that part of the gene has been artificially destroyed by Amherst!

How is it possible to find this? The method Amherst said is obviously useless!

No wonder even the talented CDC is unable to do anything about it.

This Amherst has no intention of leaving anyone a way to survive!

Lisser, who was vaguely aware that he had been tricked, patiently wanted to hear the fourth method. Maybe the fourth method could really work?

“…The fourth method is even simpler and more direct, and it’s called nucleic acid vaccine.

This method does not require any biological carriers such as live viruses, but can directly insert the relevant S protein gene fragments into the human body.

The human body's own protein synthesis engineering can be used to produce S protein, stimulate the immune system, and produce usable antibodies.

These are the four ways to develop a vaccine.

As for what you said about the methods for infected people, even if there is a vaccine, it will have no effect on them, not to mention that the vaccine still lacks the most critical S protein gene information.

Speaking of which, I’m really a god…”

boom!

A bloody rear molar flew up high and landed on the ground with a clang.

Reese was so angry that he could no longer bear it and punched Amherst hard in the face.

With half of his face swollen, Amherst spit out a mouthful of saliva mixed with blood and said with difficulty:

"Hey, you're so irritable. I didn't say there was no way to develop a vaccine."

Reese, who was about to raise his fist to strike again, grabbed Amherst's collar and lifted it up together with the iron stool beneath him.

"What did you say?!"

Amherst chuckled, he was already convinced that these guys in front of him were not really determined to kill him.

"I said there is still a way to develop a vaccine."

Rise, who was already desperate, suddenly burst out with hope:

"But you have destroyed the gene fragment of the poliovirus S protein!"

"Yes, I destroyed it, but I didn't say I couldn't recreate it.

Didn’t you take the level four virus stored in the undersea laboratory?

Just give me a laboratory and I can recover the new poliovirus, this time without destroying the S protein.

But if you hit me again, there will be nothing left."

Hearing this, Reese let go of his hand and let Amherst and the iron chair fall heavily to the ground.

There was a clang, and Amherst was hit by the force of the iron chair's repulsion, causing his buttocks to hurt.

Looking at the grinning Amherst, Reese was silent for a while and then said:

"You'd better not lie to me, otherwise I will make you regret coming to this world."

A strange smile appeared on Amherst's swollen face:

"Hey, you saved my life, do you think I would lie to you?"

"It better be so!"

Reese turned and walked out the door, leaving Frank inside looking at Amherst.

Because the box of Level 4 virus was in Du Wei's hands, and because it was such a dangerous thing, he really wanted to ask Du Wei for his opinion.

When Du Wei saw Rise, whose knuckles on his right hand were a little red, arriving, he almost understood what was happening in the room.

He said directly:

"Amherst said he wanted Level 4 viruses to be recombined to create a vaccine, right?"

The surprise in Rise's eyes flashed away. Du Wei seemed to have known this result for a long time?

But thinking of Du Wei's previous performances, Riise quickly accepted this fact.

He nodded and said:

"According to Amherst, there is no cure for the poliovirus currently circulating, unless he discovers a new poliovirus..."

After listening to Rise's explanation, Du Wei asked with a rare serious expression:

"Then let me ask you, do you trust him?

Do you know what a terrifying weapon the Level 4 virus will become in his hands?

He is humanity's top virology expert, and he is familiar with the genetic fragments of every Level 4 virus.

He has the means to arbitrarily combine the genes of level four viruses.

If he wants, he can instantly turn his laboratory into a forbidden area of ​​death where no one can enter.

So have you really thought through the consequences of giving him a Level 4 virus?"

Why doesn’t Lise know what Du Wei said?

It's just that there are still 20,000 children out there suffering from the tremendous psychological pressure caused by paralysis.

And there are many more potential children who will be affected.

In such cases, vaccines are the only barrier that can protect them from attack.

Without this barrier, Riese clearly understood that the number of infected people would not stop at 20,000.

It is bound to sweep through all new generations in New York State.

This would be a terrible 'scorched earth' plan!

"I want to take a gamble."

Facing Rise who made his choice, Du Wei shook his head and sighed.

Gamblers never end well, because if gamblers could win money, would there still be casinos in this world?

Everyone thinks they are the lucky one.

In fact, in the end, those people will only become the homeless people who say, "I used to own a big villa like you."

Now, a huge gamble on human life is placed in front of Rise, but he chooses to give it a try?

This is really a stupid choice.

Du Wei, who was about to refuse, suddenly saw Finch walking in. From the tangled look on Finch's face, it was obvious that he had been listening for a while.

"Let him try it. No matter how bad it is...it won't be too bad."

Du Wei sighed softly. It seemed that most people in this base wanted to give it a try.

No matter what, people always have to grow from mistakes.

Although the cost of this mistake will be somewhat high.

After hesitating for a moment, Du Wei finally stepped aside and allowed Riise to take away the refrigerator containing several level 4 viruses.

Looking at Rise's retreating back, Duwei said to Finch:

"Do you know that based on the information I have gathered, there is a 90% probability that Amherst will conduct more powerful virus research instead of choosing to make a life-saving vaccine?"

Finch forced a smile:

“Isn’t there a 10% chance that it’s a real vaccine?”

Du Wei shook his head again:

"Didn't you hear that I said ninety percent just to be less absolute?

In fact, I think Amherst wanted these Level 4 viruses just to upgrade the currently circulating viruses.

Personally speaking, the probability is as high as 99.9.

Unless Amherst really has a conscience and is ready to repay Reese for saving his life.

So, if you don't want New York to become a dead city, I suggest only selecting a subset of people for the experiment.

At the same time, I recommend that you wear level four protective clothing to avoid being infected by the same virus."

Finch hesitated for a while, and finally nodded.

Now is the time to race against time for virus defense. If you select a few people for security testing, you are actually giving up more people.

But even though Finch chose to take a gamble, he was not a fool.

He naturally understood that Du Wei's method was the safest method.

Otherwise, when the new virus really spreads in New York, he and Risse will really become criminals.

After Finch's confirmation, Du Wei said nothing more.

At least under his suggestion, the death toll would be fewer than expected.

Du Wei then walked to the fourth-level laboratory with transparent observation glass that was specially set up in the secret base.

This well-equipped level 4 laboratory uses a lot of Winston's gold coins.

Originally, this laboratory was prepared for Walter and Caesar, but now it is being used by Amherst.

After all, in addition to the vaccine that might protect against the polio virus, Du Wei also needed him to help him make a more virulent virus to deal with the elders of the Gaotai Table.

Amherst, who was alone with Rise in the laboratory, naturally noticed Duwei standing outside.

When he saw Du Wei's figure appearing outside the window, he felt the pressure doubled.

He didn't know if Du Wei had discovered his inner plan while standing here.

But it doesn't matter. Now that the Level 4 virus has been sent in, it's too late even if Du Wei wants to stop it.

The time it took Du Wei to pass through the four gates was enough for him to complete the virus gene replacement.

In fact, Du Wei stood here for no other reason than to learn the virology knowledge he lacked.

The 360-degree cameras scattered throughout the Level 4 laboratory can display all operations in front of Du Wei's eyes.

As for the reason why he stood outside the glass window, it was just in case.

The virus can break out in New York, but it must not break out in this base.

He doesn't have any extra gold coins to build a fully equipped underground secret base.

But having said that, if a virus that is fatal upon contact is placed in the computer room of a machine, does that mean the machine is directly in a pseudo-invincible state?

Du Wei shook his head, gave up on this bad plan with many weaknesses, and focused on learning Amherst's techniques.

Amherst is very confident in his professional field.

Almost as soon as the meal was over, Amherst made an 'ok' gesture to Reese.

Then he carefully pulled it in with a needle, and finally handed the needle into Rise's hand.

"This is the gene fragment that holds the S protein of poliovirus.

Just inject it into healthy children to produce antibodies that can resist the virus."

Amherst pointed to the new poliovirus he had reassembled and said.

Rise looked at the needle in his hand and remained silent. He was determined to use his own body to see if this so-called vaccine was useful.

But this virus only targets young children and has no effect on an uncle like him who is over thirty years old.

After restraining Amherst again, Reese took a deep breath and walked out of the room.

At this time, his hand holding the needle was trembling.

Because even if he wanted to try it, he could only use a healthy child to test whether the vaccine was effective.

If he fails, his hands will be stained with blood that will never be washed away.

He kept telling himself in his heart that this was to save more children, but even so, his psychological pressure continued to get heavier.

Until he saw Du Wei on the abandoned platform at the entrance of the base.

"You know what? If this vaccine doesn't work, kids all over New York State will be in trouble.

In other words, they just end up dying early and dying late.

And you are working hard to save them, and you are saving people who are destined to die.

Thinking about it this way, does it make you feel better?"

A smile uglier than crying appeared on Riise's lips:

"No, when you say that, it makes me feel that my back is even harder to straighten."

Du Wei spread his hands:

"Then you can also choose not to use this vaccine. Anyway, in my opinion, it is more likely that the vaccine will contain poison."

Rise shook his head firmly:

"I need to give it a try anyway, like you keep saying.

The possibility of saving one person's life by saving ten thousand is worth our efforts no matter what.

So if you fail, you will lose your life.

But if we are lucky enough to succeed, we can save tens of thousands or even hundreds of thousands of children.

It’s a good deal.”

Rise felt inexplicably refreshed as he spoke out what was in his heart.

Seeing the smile on Du Wei's face, Rise realized that Du Wei was using this method to guide himself to release his stress.

He is indeed feeling much better now.

I won't be as lost as I was just now.

"Okay, let's go.

If successful, remember to hand the person over to the Centers for Disease Control and Prevention. They can find a way to develop a vaccine in batches from the antibodies in the person.

If it fails...then burn the body, high temperature is a good way to prevent the virus from spreading again."

Reise nodded and walked towards the tunnel in the darkness with firm steps.

When Rise's back was no longer visible, Duwei walked to the room where Amherst was being held.

Amherst looked very surprised when he saw Du Wei arriving. His first question was:

"Are you here to kill me?"

Du Wei shook his head:

"No, you are still of great use to me. You are not allowed to die before I squeeze out all your remaining value."

Amherst chuckled:

"Even if I'm about to kill all of New York City?"

"Even if you're about to kill the entire city of New York."

Amherst was stunned for a moment, then laughed and said:

"Ha, I didn't expect you to be such a cold-blooded person."

The corner of Du Wei's mouth curled up slightly, and he picked up a willow leaf knife and a blood-stained fishing net from the torture tool bag beside him.

Then, under Amherst's surprised gaze, he used a fishing net to pull out the meat on his thigh, and then used the willow leaf knife to lightly skim it.

A thin piece of meat flew out accompanied by Amherst's scream.

"Yes, so I want you to immediately develop a level five virus for me that will kill you if you touch it.

Otherwise, I will let you know what I will do in cold blood."

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