I cultivate mushrooms in warhammer

9 PM, a simple and cramped dwelling.

The room was filled with materials related to cultivating fungi, making it even more crowded than before.

Li Nuo sat on a chair, silent, immersed in the task of simulating the taming of a simpleton mushroom.

【Day 8, all samples are contaminated by miscellaneous bacteria. This simulation has failed.】

Failed again.

Damn it.

Is the vitality of miscellaneous bacteria in the Warhammer world this strong?

Even alcohol and sunlight can't kill the miscellaneous bacteria on the simpleton mushroom, that's too outrageous.

What's even more outrageous is the simpleton mushroom itself.

This thing looks similar to ordinary poisonous mushrooms, but it's surprisingly durable, able to grow even when soaked in alcohol.

It's simply outrageous to the extreme, outrageously outrageous.

Li Nuo took out a milk mushroom and started munching on it to relieve the bitterness in his heart.

It seems I have to change the method of taming.

Now I have a lot of money on hand, let's spend a bit and buy the sterile tools needed for the serial dilution plating method.

Li Nuo crawled under the bed, took out a large stack of ration cards, hid them under his clothes, and went out.

It was already 11 PM when he returned home.

Besides the fatigue from a busy day, Li Nuo's face also showed a hint of heartache.

He had a black leather suitcase with a mechanical combination lock in his hand.

He spent nearly three hundred ration cards for the things in the suitcase, which can be described as a major bleeding.

Hopefully, this simulation will be successful.

Putting down the suitcase and unlocking the combination lock, a glass container appeared before him.

Inside the container were sterile test tubes, sterile droppers, sterile Erlenmeyer flasks, sterile spreaders, sterile culture medium plates, and a pair of gloves for performing sterile operations inside the container.

This set of equipment is called a portable sterile chamber, emphasizing simplicity, without ultraviolet light or a fan.

It was a second-hand item that Li Nuo had picked up from the market. The original owner was an unlicensed wild pharmacist.

This person had recently invented a special medicine for treating trauma, making a lot of money and buying many new devices, selling his used items at a discount, which is how Li Nuo got such a good deal.

The portable sterile chamber is not the ideal experimental equipment.

What Li Nuo really wanted to buy was an ultra-clean workbench.

It can form a positive pressure state inside and use ultraviolet light to sterilize, thereby creating a sterile environment.

However, ultra-clean workbenches are too expensive, so Li Nuo had to settle for second best and use a portable sterile chamber as a substitute.

Normally, before using a sterile chamber, you must first ensure that there are no miscellaneous bacteria in the surrounding environment.

Otherwise, when putting the sample in, the sterile chamber will be contaminated by the miscellaneous bacteria in the environment.

The current residence obviously does not meet the conditions for using the sterile chamber.

But it doesn't matter.

The system has helped a lot in this regard.

Li Nuo reached out and pressed on the sterile chamber, and the system interface popped up.

【Portable sterile chamber detected.】

【Absorbing it can permanently upgrade the fungal simulation function.】

【Do you want to absorb it?】

Li Nuo silently recited "Absorb".

“Whoosh — — — —”

After the ethereal prompt sound, the sterile chamber disappeared out of thin air, and the suitcase was empty.

【Fungal simulation function upgraded successfully.】

【New simulation container added: Portable sterile chamber.】

【From now on, you can choose this container for simulation without preparing additional containers.】

Li Nuo sat up straight:

“Start simulating the removal of miscellaneous bacterial contamination.”

【Simulation is about to begin.】

【Please select the experimental object.】

“Spores of the simpleton mushroom.”

【Experimental object set.】

【Please select a container.】

“Use the sterile chamber and the sterile tools inside the chamber.”

【Container set.】

【Please select the culture medium and nutrient solution.】

Li Nuo gestured at the table, where the raw materials were placed:

“Use the formula I used in the previous simulation to make the culture medium and nutrient solution for this simulation.”

【Please set the simulation parameters.】

“Use the serial dilution plating method to separate and purify the spores contaminated by miscellaneous bacteria. Refer to the previous simulation records for parameters such as temperature, humidity, and light.”

【Set.】

【Start simulation.】

【Day 1, you use the spores of the simpleton mushroom and an Erlenmeyer flask containing sterile water to make a 10⁻¹ bacterial suspension.】

【You use the 10-fold serial dilution method to obtain a 10⁻⁶ bacterial suspension.】

【You use a sterile spreader to spread the diluted bacterial suspension on a sterile solid culture medium plate.】

【You harvest 10 experimental samples.】

【You observe the samples statically.】

【Day 2 to Day 5, microorganisms on the culture medium plate grow and reproduce, forming colonies.】

【You use the analyze fungus function to determine the types of microorganisms in the colonies.】

【You find that all 10 experimental samples are contaminated by miscellaneous bacteria.】

【The least contaminated sample has colonies composed of 33% miscellaneous bacteria and 67% simpleton mushroom mycelium.】

【At this time, you decide?】

The principle of the serial dilution plating method is easy to understand.

Suppose there are 99 miscellaneous bacteria and 1 fungus you want in 100 microorganisms.

We use the dilution method to mix these 100 microorganisms with a large amount of sterile water to obtain a bacterial suspension.

Divide the bacterial suspension evenly into 100 portions of bacterial suspension with lower microorganism concentrations.

If you're lucky, one of them will have no miscellaneous bacteria.

Take this portion out separately and cultivate it carefully to obtain a fungus that has not been contaminated by miscellaneous bacteria.

This is the general process of the serial dilution plating method.

What if every portion of the bacterial suspension has miscellaneous bacteria?

Don't panic.

Continue to dilute, continue to divide evenly, and continue to perform the serial dilution plating method until a bacterial suspension without miscellaneous bacterial contamination appears.

This method of removing miscellaneous bacteria is very cumbersome and often takes several months for the experimenter.

But it is undeniable that as long as you persevere, you will definitely get a non-contaminated bacterial suspension.

Li Nuo didn't hesitate much and instructed the system:

“I choose the least contaminated sample as the new experimental object and use the serial dilution plating method again for separation and purification.”

【Day 5, you use the serial dilution plating method on the new experimental object to obtain 10 experimental samples.】

【Day 10, you use analyze fungus on the samples and find that the least contaminated sample contains approximately 30% miscellaneous bacteria, with the remainder being simpleton mushroom mycelium.】

【At this time, you decide?】

Hmm, very good, the contamination level has decreased from 33% to 30%, which is good news.

“I decide to repeat the previous actions and continue to separate and purify the least contaminated sample.”

【Day 10, you obtain 10 new experimental samples.】

【Day 15, the least contaminated sample has approximately 28% miscellaneous bacteria. At this time, you decide?】

“Continue.”

【Day 20, the least contaminated sample has 26% miscellaneous bacteria.】

“Continue.”

【Day 25…】

【Day 30…】

【Day 35…】

The number of simulated days rose rapidly.

Li Nuo sat on the chair, repeating his instructions over and over again.

Soon, the time came to midnight at twelve o'clock, but there were still 2% of miscellaneous bacteria that had not been separated.

Li Nuo remained calm and did not give up.

【Day 160.】

【Day 380.】

【Day 620.】

How stubborn were the remaining miscellaneous bacteria? The system had performed no less than a hundred separations and purifications, and there were still less than 0.02% of miscellaneous bacteria in the sample.

The miscellaneous bacteria in the Warhammer world are really too stubborn!

Finally, when the number of simulated days reached a terrifying one thousand two hundred, Li Nuo finally got the result he wanted.

【Day 1200, you have obtained a sample that has not been contaminated by miscellaneous bacteria.】

【You find the mycelium of the simpleton mushroom in the sample and cultivate it.】

【Day 1208, you harvest the sample and obtain 15 tamed simpleton mushrooms.】

【Congratulations!】

【The simulation plan has been recorded.】

【Do you want to consume the simulated materials to obtain 15 tamed simpleton mushrooms?】

“Finally succeeded! If this were before I transmigrated, my hair would have turned white from staying up so late!”

Li Nuo was so excited that he couldn't add anything, and hurriedly silently recited "Confirm".

Fifteen simpleton mushrooms appeared in the fungus warehouse, all snow-white and without any variegation.

Li Nuo looked at the clock on the wall.

Two o'clock in the morning.

Hurry up and try the effect of the brain zombification technique, and then go to sleep.

Otherwise, I might oversleep at the excavation site during the day.

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