I Exchange For Black Technology By Blowing Up The Bully
Chapter 131 Selecting Engineering Bacteria
There is only one place!
The high school students looked at each other apprehensively, as if their friends turned into enemies in seconds!
Having been in the high-pressure fight of competing teams for a long time, they have already developed a conditioned reflex for the competition for places.
What's more, the first laboratory is luxurious and majestic, everyone dreams of it. Those advanced equipment costing hundreds of millions are like a group of peerless beauties and handsome men. If you don't want to be obscene, even if you can touch it with your own hands, the refreshing feeling is unimaginable!
Therefore, Yi Chunxiao's words hit everyone's heart!
Only Song He was very calm and not hostile to his classmates.
It wasn't that he didn't want to go to the first laboratory, nor was he friendly to others, it was simply because he was always invincible and didn't take the students here seriously.
"Okay, let's do it!" Yi Chunxiao said.
All the students got busy immediately, adjusting the experimental equipment, cleaning the tools, and racing against time to make preparations.
Everyone moves fast. After all, this is not only a test of knowledge and operation, but also a contest of time!
While Song He was busy, he looked around like an old fox, spying on other students who were preparing their culture medium.
Many test benches have boiled water to process agar, which looks like jelly, but is actually used by food factories to make jelly.
The students were busy putting it on an asbestos net, boiling it and melting it, and when it was about forty degrees, the agar would solidify and be used as a coagulant for solid medium.
Look at the few things in the raw material box, almost all students are equipped with LB medium.
As expected! Song He couldn't help but sneer.
LB medium is the most common formula for cultivating Escherichia coli. It is the abbreviation of the word "lysis broth" in English. The specific content is 10 grams of tryptone, 5 grams of yeast extract, and 10 grams of sodium chloride, dissolved in 1 liter in deionized water.
However, although the engineered bacteria are transformed from E. coli, they are not pure E. coli. Using LB medium, they have fallen behind from the beginning!
The culture medium configured by Song He is slightly more complicated, and it is a variant of SOC medium, which is relatively unpopular.
There is more glucose in his medium, which can act as a catabolism inhibitor. The engineered bacteria will be more inclined to eat glucose to extract carbon sources, thus ignoring the decomposition of other carbon sources. Inhibits the lactose operon, reducing foreign gene expression.
The formula is tryptone, yeast extract, sodium chloride, potassium chloride, magnesium chloride, magnesium sulfate, dextrose. To be on the safe side, a little more glycerin was added as an extra carbon source.
In formulating a unique culture medium, Song He is actually gambling.
He was scared and excited at the same time, wondering if he could give a chance to turn his bicycle into a motorcycle?
In the end, he did not use solid media, but liquid media.
He must observe the movement of engineering bacteria all the time and adjust the formula of the culture medium. It is more convenient to use liquid, but the risk is also greater.
"Song He, have you finished reading the information book?" Xu Tingfeng asked in a low voice.
"It's over." Song He nodded, his hands moving quickly.
"Understand everything? Do you understand the characteristics of each engineered bacteria?" Xu Tingfeng looked expectant.
"Roughly the same."
"Can you tell me which number of engineering bacteria is best to cultivate?" Xu Tingfeng was excited.
"Please." Song He smiled.
[Xu Tingfeng has a little resentment towards you, resentment value +80! 】
"Please, you are the most talented, tell me!" Xu Tingfeng blushed slightly, she seldom begged for others, and flattering was particularly unfamiliar.
"Isn't this the first sister of the biological team? Why are you so lazy?" Song He cocked his mouth in a strange mood.
[Xu Tingfeng feels that you have ridiculed him, resentment value +200! 】
"What's the number of the bacteria?" Xu Tingfeng jumped anxiously, "Someone has already gone to collect the bacteria!"
"Please don't talk about it, you are responsible for bringing me meals for the past two weeks. I have been spending time in the laboratory and have no time to queue up in the cafeteria." Song He said.
"Okay, okay! I'll bring you food!" Xu Tingfeng softened.
"There are a total of 40 kinds of engineering bacteria, all of which are waste bacteria." Song He lowered his voice and said in a firm tone, "Other bacteria will die after a period of time with conventional cultivation methods. Only No. 33 can be directly cultivated. The reason why No. 33 is invalid is There are too many impurities, but there is no problem with its own growth and reproduction.”
"Thank you!" Xu Tingfeng ran away instantly to get the engineering bacteria.
Song He took his time and put the prepared liquid culture medium into the machine, set a high pressure of 15psi, and started steam sterilization.
The culture medium must be clean. In case there are any strong bacteria left in it, grab the engineering bacteria and eat them as jelly beans, and the experiment will be sent.
Cultivating fungi is like raising a child. First of all, you must make sure that the child is your own, and don’t be greened by other fungi. You have worked so hard to raise a bunch of other people’s children, and you have the heart to die.
After it was dealt with, Song He followed his classmates to line up.
He was a little slow, the last one.
At the entrance of the first laboratory, a senior and a senior were sitting at a table filled with sample test tubes.
The senior is Fan Tao, wearing a mask, only revealing a pair of beautiful fox eyes, looking at the students in line with a sneer, a high-ranking imperial sister Fan.
"Oh, what a smart choice to choose number 12!"
"Number 22, I wish you success! Poor boy!"
Fan Tao handed out the test tube of engineered bacteria, while talking with a smile, his tone was full of sarcasm.
The senior is Xiong Xiuwei, who is dark and strong, like a blind Xiong who has cultivated into an adult, and speaks in a rough voice.
"Choose number 6? Little classmate, you are an idiot!"
"Number 40? Do you have Coke in your head? Half water and half bubbles!"
Xiong Xiuwei's sarcasm is more direct, and he also laughs, which is quite annoying.
The high school students silently endured the malice of their seniors and sisters, felt wronged and angry, and left the test tube tightly.
Song He decisively lined up with Fan Tao, neither of them was good, at least Senior Fan Tao was pretty enough.
In front is Xu Tingfeng.
She was uneasy, watching the other students take away the engineering bacteria one by one, and struggled fiercely in her heart.
In fact, she took a fancy to bacteria No. 14 and thought there was nothing wrong with it.
But Song He said that only bacteria No. 33 is fine, is he telling the truth?
14, 33, 14, 33... In Xu Tingfeng's mind, it seemed like two villains were fighting.
Finally, it was her turn.
"What number is the bacteria?" Fan Tao asked casually.
"No. 14..." Xu Tingfeng suddenly changed his words, "No! No. 33!"
Fan Tao's eyes just filled with sarcasm, but suddenly his eyes changed, he looked at Xu Tingfeng carefully for a moment, and handed her the test tube No. 33.
Xiong Xiazi's senior, Xiong Xiuwei, was stunned when he heard 33, and looked at Xu Tingfeng seriously, with a flash of surprise and approval in his eyes.
Quite abnormally, neither of the two seniors made any mockery, but remained silent!
Xu Tingfeng keenly sensed something was wrong with the atmosphere, and was overjoyed!
Song He didn't lie to me! No. 33 is a good fungus! At least it is better than those chosen by the previous students!
She didn't leave immediately, but stood aside and waited.
Everyone is in a competitive relationship. Song He must have kept the best bacteria for himself. I am afraid that number 33 can only be regarded as the second best. She was curious what number Song He would pick.
"What's the date?" Fan Tao narrowed his eyes and looked at Song He.
"No. 1." Song He said very bachelor, as if giving up on himself.
Fan Tao and Xiong Xiuwei were taken aback for a moment, and then burst out laughing!
"It's really a fool to choose No. 1!" Xiong Xiuwei kept laughing, tears were about to come out, "When I compiled the information book, I deliberately put the worst No. Die fast!"
"I'll give you a chance to choose another one." Fan Tao sneered, "All bacteria can be selected, and there are many samples. It's not that if someone else chooses you, you can't choose."
"Number 1." Song He knocked on the table, urging, "So much nonsense! Hurry up!"
"If he wants number 1, give him number 1! Let's see how long it lasts!" Xiong Xiuwei laughed.
"The two of us are going to do experiments. If we want to get the bacteria, we have to wait until we are free. It may not be when we are free. When you kill all the bacteria, just wait!" Fan Tao took out the No. 1 test tube, His shoulders shook with laughter.
"Laugh, you won't be able to laugh when I feed No. 1." Song He took the test tube.
He turned and left, returning to the test stand.
Xu Tingfeng followed, confused.
"Song He, what are you doing! Why did you get the worst No. 1? No. 1 died after not being able to live for two hours! With your level, you can't see it, right?"
"You pick a healthy fungus and feed it. It's very simple, and you can't learn anything." Song He replied calmly, "The more disabled the waste fungus, the more challenging it is to feed it!"
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