My Life Simulator

Since the virtual gene chip, the progress of the experiment has accelerated significantly.

The detection of gene expression information is almost like making a puzzle. It was very difficult at the beginning, but as more and more gene fragments were identified, the speed became faster and faster.

Seeing the progress of the experiment in the virtual lab, Lu Li nodded slightly. According to the current process, the human genetic map should be completed within a few years.

Genetic testing continues, but gene-targeting technology is at a loss.

Before the completion of the human genetic map, it is really difficult to improve the gene positioning from the software, that is, the accuracy of the information.

Maybe... there is something wrong with my thinking.

Since the ability of transcriptase is not good, then change to another ability!

When his thoughts turned to here, Lu Li suddenly became enlightened.

Transcription activators, like effectors (TALEs), with gene targeting specificity in human cells, are not irreplaceable!

There must be other undiscovered "enzymes" that can replace the function of transcriptase.

So Lu Li began to study various cells, bacteria and viruses.

Animal cells, plant cells, bacteria, and even viruses, analyze cellular structures for enzymes with gene targeting specificity.

The process was not smooth.

Finding enzymes with target specificity in a wide variety of protein molecules is a matter of chance.

Day by day, enzymes with target specificity remain undiscovered.

Liu Qin still came to Fudan. As in reality, Liu Qin became an associate professor at Fudan School of Economics.

It's just... The difference from reality is that before Liu Qin joined Fudan, he accepted a strict and meticulous political review.

Because when Liu Qin was a Ph.D. student at Harvard, interpersonal relationships were relatively simple, and there were no suspicious social relationships. Moreover, Liu's father's business is also based in China. He has no foreign assets at all, and he has no business dealings with foreign countries.

So... Liu Qin's political trial is no problem.

When Liu Qin told Lu Li the news of the political trial, Lu Li was helpless. It turns out that when I fall in love with someone now, I still need to go through state censorship.

The arrival of Liu Qin has enriched Lu Li's weekends, and he no longer has to go to the laboratory every weekend.

Professor He is very pleased with this. Scientists are also human, and they cannot regard themselves as machines. Maybe the arrival of love can inspire more inspiration.

This...it turned out to be true!

In the second month after Liu Qin joined Fudan, Lu Li's research finally made a key breakthrough.

He found another enzyme with target specificity - nickase!

Among the type II restriction enzymes, there is also a special type. The enzyme only cuts one strand of the double-stranded DNA, resulting in a nick. This type of restriction enzyme is a nickase.

From a functional analysis, transcriptase is a large-scale search radar, and nickase is a fire control radar.

In terms of positioning accuracy, the nickase is indeed very accurate, almost every shot, the problem is... without a large-scale search radar, it is difficult for the fire control radar to find the target.

That being the case...then combine the two.

So, Lu Li started experimenting.

Prepare cleavage protein, prepare transcriptase, prepare nickase, and then combine the three to form a brand-new gene editing tool.

Next, Lu Li entered the guide RNA on the transcriptase to determine the characteristics of the target gene. Then use the gene gun to inject the new gene editing tool into the cells to be edited.

Under the electron microscope, the guide RNA on the transcriptase begins to search for the target on the gene chain, and the guide RNA matches the target gene to initially lock the approximate orientation of the target.

Immediately, the spliced ​​protein begins to move towards the target orientation.

When the shear protein moves to the position of the target, the nickase fire control radar is turned on, the accurate target is instantly calibrated, and the target is locked!

In the next instant, the spliced ​​protein moves to the target position and begins to fuse with the gene chain, and the helical gene chain is unwound and flattened.

Then... as if a "click", the shear protein accurately cut the marked position.

Good start! The first experiment was successful!

Afterwards, Lu Li repeated the same experiment hundreds of times, and the results of each experiment were all 100%, without the slightest error!

It worked!

The combination of scanning radar and fire control radar allowed Lu Li to create a brand new gene editing tool.

Revolutionary breakthrough in gene targeting technology!

Since then, human gene editing technology has reached a new level.

The new gene editing tool can accurately lock the target and cut the required gene fragment accurately, no longer need to take luck.

Lu Li closed the virtual lab with a bright smile on his face.

Another major breakthrough has been made in genetic technology!

Back in the study, Lu Li sat at the computer desk and began to write the paper.

"Gene targeting technology, a new gene editing tool."

In the paper, Lu Li described a new gene editing tool with meticulous logic, rigorous presentation and detailed experimental data.

In the original cleavage protein + transcriptase gene editing technology, the nickase "fire control radar" has been creatively added, which makes this brand-new gene editing technology have a very high gene targeting accuracy, and the gene editing efficiency is high. A thousand-fold increase.

After writing the thesis and printing it out, Lu Li walked out of the office with the thesis materials.

"Teacher, I have written a new paper, take a look at it."

When he came to Professor He's office, Lu Li handed over the thesis materials in his hand.

"New paper? You... have the results again?"

Professor He sighed inwardly, "It's so terrifying", and reached out to take the paper handed over by Lu Li.

"Gene targeting technology? Brand new gene editing tool?"

Seeing the title of the paper, Professor He took a breath of cold air, uh... the cold air from the air conditioner!

Did Lu Li come up with a brand new gene editing tool?

Suppressing the shock in his heart, Professor He looked down at the thesis materials in his hands.

After reading it carefully, Professor He has clearly seen from the statement of the paper that a gene editing technology with higher efficiency and higher accuracy has been officially born.

With this new gene editing tool, human gene editing technology has reached a new level.

Lu Li...he really is a genius!

First came up with a new anti-cancer technology, and now a new gene editing tool has been developed.

The pillar of the state, the blessing of the community!

Lu Li...will definitely lead the future!

Looking up at Lu Li in front of him, Professor He suppressed his excitement and said to Lu Li, "Go! Let's do a verification."

"OK!"

With that said, the two walked out of the office together and came to the laboratory.

In the laboratory, Lu Li repeated the experimental process in the virtual laboratory again, and performed one hundred gene editing experiments in a row.

The success rate is 100%, and the accuracy rate is 100%.

"Amazing! It's amazing!"

Professor He picked up the verification data in the laboratory, his hands were shaking, "The efficiency of gene editing has been improved thousands of times. This is a revolutionary breakthrough!"

Lu Li smiled.

"this essay……"

Professor He raised his eyes to look at Lu Li and said seriously: "Delete the specific experimental steps, delete the detailed experimental data, just make a theoretical statement, revise it, and send it to the top international academic journal 'Nature'."

Speaking, Professor He looked at the paper in his hand again, and said, "I submit this paper to the Chinese Academy of Sciences as an internal document."

"OK!"

Lu Li agrees with Professor He's practice of "keeping one hand".

Science has no borders, but scientists have nationalities!

We master the latest technological achievements by ourselves, as for others... give you a direction, go and study it yourself!

Later, Lu Li revised the paper and wrote a directional and theoretical paper. It's just... At the end of the paper, Lu Li attached the laboratory's verification conclusion.

After submitting the manuscript to the journal "Nature", Lu Li posted his paper on the academic website again.

...

across the Pacific.

In the Millikin Institute of Genetics, researchers are working intensely and busy.

In the research of genetic technology, the Millikin Institute of Genetics has enough confidence that they have been at the forefront of the world.

The concept of gene chip technology was proposed by them.

The gene editing technology CRISPR-Cas9 was also developed by them.

Although Lu Li's new generation of anti-cancer technology has achieved success, it is only a result of technical application, and the foundation is still CRISPR-Cas9 gene editing technology.

It's just... CRISPR-Cas9 technology, the efficiency is still too low!

Gene mapping is inaccurate, gene editing is off-target, and thousands of experiments are required to obtain a correct specimen.

So... the chief scientist at the Millikin Institute for Genetics, Steven, is not happy with that.

"We are looking for a new gene editing tool. I will solve the funding problem. Even if it costs tens of billions of dollars, we must develop a new gene editing tool within three years. We... Lead the world!"

At this morning meeting, Dr. Steven's voice was impassioned.

Then...his speech was interrupted by a scream!

"I buy it!"

A man with thinning hair and a deep hairline looked at the screen of his mobile phone and exclaimed: "Dr. Steven, you'd better look at the academic website first! Someone has developed this new gene editing tool. !"

"what?"

Dr. Steven was taken aback for a moment, then quickly took out his mobile phone, opened the academic website, and saw a paper that caused a heated discussion.

"Gene targeting technology, a new gene editing tool."

Author... Lu Li!

It's him?

Steven's heart skipped a beat, and he hurriedly opened the paper and read it.

The paper theoretically describes a new combination of gene editing tools, splicing proteins, transcriptases, plus a new "targeting enzyme".

Targeted by "targeting enzymes" to achieve accurate gene editing.

This kind of theoretical paper is actually not of much value, and it is almost enough to look at it.

However... At the end of the paper, Lu Li even attached a laboratory verification report, confirming that the accuracy rate of the new gene editing tool is as high as 100%!

This proves that the new gene editing tool developed by Lu Li has been successful!

But... what kind of enzyme is this "targeting enzyme"?

Falco! Why not write down what enzyme is it?

Dr. Steven jumped in anger, slapped his phone on the table with a "pop", and pointed at the man with a deep hairline who exclaimed before, "You, this month's bonus is gone!"

"why?"

The man with the deep hairline had a dull expression on his face.

"Because...you were playing with your phone during the meeting!"

Well, this reason... is too powerful!

The man with the deep hairline pouted slightly, Dr. Steven, you should be outraged, right?

...

Lu Li's paper, which has not yet been officially released, has already caused a violent response in the academic world.

Countless people watched that "targeted enzyme" gnashing their teeth. What exactly is a targeting enzyme? Why did you write it for me!

There are tens of thousands of known enzymes. There are countless variants of each enzyme. If you don't write it out, how do we find this targeting enzyme?

Science knows no borders, why should it be so narrow? Announce it! Publish it to me!

...

The next night, another news about Lu Li was broadcast on Huaxia TV's news network.

"News from this station: Dr. Lu Li, a famous scientist in my country, has developed a world-leading scientific research achievement after conquering cancer, and created a new gene editing tool. The gene editing accuracy rate is as high as 100%, and the gene editing efficiency is improved. Ten thousand times."

"The success of this technology research marks that my country has reached the world's leading level in the field of gene editing."

After the news was broadcast, people watching TV had only one feeling in their hearts, Lu Li again! It's Lu Li again!

Online media immediately followed suit, reporting on Lu Li's world-leading new technology.

"Lu Li, the pride of the Chinese people!"

"Leading the world again, cheers for Lu Li!"

"Lu Li created a brand new gene editing technology."

"A great breakthrough in gene technology, Lu Li has made gene editing technology leapfrog."

"This man once again leads the world and leads the times!"

On TV, in newspapers, and in the continuous reports on the Internet, Lu Li's reputation has just dropped, and once again, it has become popular all over the country and is famous all over the world.

"Your surname is Lu, and sure enough, 666!"

"God, please accept my knee!"

"I'm writing a graduation thesis on 'Imagination of Next-Generation Gene Editing Technology'! You have already researched it. How can I still graduate!"

"Sympathy for the wave upstairs. Besides...how can I not help laughing out loud?"

"As a researcher in genetics, let me talk about the importance of this technology. The emergence of this technology is similar to the invention of the steam engine. Genetic engineering has entered a new era!"

"The next era, the genetic era. Lu Li led the genetic era for us!"

"Lu Li, the man who leads the times!"