Plague Doctor

Cai Zixuan looked around for a while before saying, "The leader of our group is Wang Ruoxiang."

"Is this the case?" Gu Jun frowned helplessly, thinking what was going on, "It's not surprising at all, okay, it's not her that makes me wonder."

"Yeah, that's Wang Ruoxiang." Cai Zixuan was filled with emotion.

Wang Ruoxiang is the monitor of their class, with strong ability, beautiful person and good personality. Such a school goddess, the people who pursue her are of course lined up from the teaching building to the bureau's solution building. Tuhao Jun is one of them. Back then, it was a vigorous pursuit, but in the end, Wang Ruoxiang refused to end with a stern statement in public: "I'm sorry, we are not the same. Human, I wouldn't like someone like you."

Um, a tall, handsome, rich, and eight-year-old man, no matter how bad he is, turned out to be "this kind of person"?

At that time, everyone said that thanks to the local tyrant Jun and the squad leader, they made them believe in love again.

"The squad leader is still single now." Cai Zixuan obviously didn't know how old-fashioned he looked now.

"Don't look at me like this." Gu Jun laughed at himself, "It's all over." Indeed, it's all over. The local tyrant Jun is somewhat fond of pursuing the squad leader, and a bit more because he wants to catch everyone's goddess. Those deep things don't matter.

"Really." Cai Zixuan stopped talking about the monitor and looked at his watch, "It's almost 8 o'clock, I have to go to the laboratory."

"Let's go, I'm also going to the experimental building." Gu Jun said.

Cai Zixuan suddenly showed a knowing look. He just said that he didn't care about the monitor, but now he turned his head and went to join in the fun. It's nice to be young.

"What?" Gu Jun was really helpless, he wanted to explain but didn't know where to start, "Forget it..."

"Haojun, you don't need to say it, you understand, once the sea was difficult, I understand."

"You understand a cavernous body."

The medical laboratory building is in the northwest of the campus. It is a dozen-story white-walled brick building with no distinctive appearance, and even opposite the entrance is a rotten cement floor in disrepair. However, there are laboratories with well-equipped equipment in the building for teachers and students to use in scientific research.

"This way." After entering the building, Cai Zixuan walked in front, introducing the situation to Gu Jun as he walked.

The title of the experiment done by their group is called "Several photosensitizers for photodynamic therapy of tumors". It is a study of subcutaneously implanting tumors in mice, then using different photosensitizers for photodynamic therapy, and then observing tumor changes. .

The allocated funds this time are only 30,000 yuan, which is too much for an undergraduate scientific research experiment, but an imported pure photofrin photosensitizer can cost tens of thousands of yuan. In order to make several more photosensitizers, Wang Ruoxiang made careful calculations and saved money to set up domestic imitation photofrin group, dotiporfin group, ala group, plus blank control group, a total of 5 groups of mice.

"The research plan and the opening report are all done by the team leader." Cai Zixuan couldn't help but sigh, "It is indeed Wang Ruoxiang."

Although Professor Gu is a mentor, he only occasionally takes the time to give guidance. For a big man like him, it is his business to score high scores in sci articles. Therefore, the Pioneer Cup still depends on the students themselves. The team leader does the most things, but it is an opportunity that everyone can't ask for.

Because if the experiment results in a paper, Wang Ruoxiang is the first author, and Cai Zixuan and the others can only rank behind.

"We are still in the first stage, that is, to establish an ascites tumor-bearing mouse model, which has been cultivated for a week. Today, it is considered impossible."

Cai Zixuan said a lot, and suddenly found that Gu Jun didn't speak for a long time, and seemed to frown when he heard the word "tumor"...

"Uh Haojun... You know, the success rate of modeling is not 100%."

Cai Zixuan scratched the front bald part of his head, he really didn't know what he said just now, did Gu Jun understand.

"What in the world can be 100%." ​​Gu Jun lowered his head and covered his face, friend, I haven't learned enough for that.

"Indeed." Cai Zixuan was very touched by this sentence, and a look of vicissitudes appeared on his face, "Everything has something to do, like a dream bubble."

Well, Gu Jun doesn't understand...

During the conversation, the two took the elevator to the eighth floor, where the laboratory used by their group was located.

Walking along the corridor, Cai Zixuan opened the door and walked in first when he reached the door of the laboratory, only to see that all the team members had arrived, and he was the last one.

"Zixuan, I'll be waiting for you."

"what?"

At this time, everyone saw a person who walked in later, and the laboratory suddenly fell silent, leaving only the chirping of mice.

The local tyrant...?

Didn't he say he dropped out to travel the world? Didn't he say he hung up? Not that he...

"Hello everyone." Gu Jun waved to everyone with a smile, looked at Wang Ruoxiang, "Hello, monitor."

Wang Ruoxiang was standing beside an experimental table. She had black short and medium hair, and her delicate melon-seed face was very good-looking despite her plain makeup. She had a slender figure and a white coat, and she was full of heroic spirit. Seeing him, Wang Ruoxiang frowned slightly, "Hello Gu Jun." The voice was also very nice.

Gu Jun nodded in response to her. After three months of separation, the squad leader is still so beautiful, but he is no longer the one he used to be.

"Hao Jun had something to do before, but he's back now." Cai Zixuan explained to everyone, and then introduced Gu Jun: Xu Hai, a clinical student, is an acquaintance, Zhang Haoran, a basic medicine major, and He Yuhan, a pharmacy major, in a group of 5 Everyone is there.

Cai Zixuan asked, "Squad leader, Hao Jun wants to have a look with us, is it okay?"

Wang Ruo

Xiang pursed her lips silently, and said, "Just stand on the side and don't do anything."

Local tyrant. She murmured in her heart, remembering that at the beginning of school a few years ago, she had a superficial affection for him... ugh. This kind of person is delicious and lazy, has all five poisons, rotten wood can't be carved, mud can't support the wall... Mice are not as good! It may only be possible to contribute to medicine if someone is pulled to slice it for research.

"No." Gu Jun obediently stood aside, but always felt that Wang Ruoxiang's eyes were not good... an illusion.

There are already a row of mouse cages on the experimental bench, and each cage has a group of white Kunming mice. This is the most commonly used experimental animal. It is very docile and small. The body weight is only 18-22g at 1-1.5 months old, and the body length is less than 10cm, so it can be used for experiments.

Except for one cage of model mice, the others were all blank mice, which Wang Ruoxiang just bought from the school's experimental animal center. Once modeled, they can come in handy.

Now that the team members have arrived, everyone immediately starts to work.

crackle! Wang Ruoxiang put on rubber gloves and went to the model mouse cage to catch the mice. These mice were numbered with staining, and she caught mouse No. 1. Cai Zixuan and a few others were in charge of assisting, recording, recording, and taking pictures.

"The eighth day of intraperitoneal implantation of fibrosarcoma in s180 mice." Wang Ruoxiang looked attentive and serious, and lightly pressed the obviously bulging abdomen of mouse No. 1 with her hand, and the mouse then let out a burst of painful chirps, She struggled, but she held it firmly in her hands.

She said: "The ascites of mouse No. 1 continues to grow. It is already a frog's abdomen. It has good vitality and does not need to be sacrificed for the time being."

Hearing what she said, the team members were immediately a little excited, and they all leaned over to see clearly, "Is this the 'frog belly'." "Amazing."

"As expected of a frog's belly, it's really big." Cai Zixuan said with emotion.

Although they are all eight-year programs, it is the first time to do this kind of real scientific research experiment.

"Oh." Gu Jun also leaned over to take a look, and still understood what they were doing.

The reason why the ascites-type tumor-bearing mice were made first was to obtain a large number of highly active s180 tumor cells. You must know that the original cell line is very expensive, but one tube is only enough to inject one mouse, and the activity is very fragile, and the four treatment groups they set up need to train hundreds of mice.

So it is first to manufacture, and then to expand. Now that the mice have grown frog abdomens, they can "receive ascites".

"Gu Jun." Wang Ruoxiang suddenly turned her head and glanced at him, "Can you walk away? I'm in the way."

"Okay." Gu Jun shrugged and walked away, your aura is okay, you can get in the way if you are a meter away.

Cai Zixuan and the others didn't see it at all, and Wang Ruoxiang continued the next step.

She pierced the abdominal cavity of the No. 1 mouse with a 5ml syringe, and successfully drew out a tube full of yellow clarified ascites, and then injected it into an eppendorf conical bottom centrifuge tube, which she took and centrifuged with a centrifuge. Immediately after centrifugation, a large amount of white sediment appeared at the bottom of the centrifuge tube, which were tumor cells.

"Mice No. 1 was successfully modeled." Wang Ruoxiang was relieved and happy, and smiled to the team members: "There are a lot of s180 tumor cells in the ascites!"

"Yeah!" "Haha." They were all young people, no one paid any attention to the mice's feelings, they cheered, Cai Zixuan praised: "Take the first step to success!"

The first step was successful, and then the cells were given to mice in the treatment group to create subcutaneous tumors, which were then treated with photodynamic therapy.

There is still a lot to do, they continue to work, remove the supernatant from the centrifuge tube, resuspend the count, adjust the cell concentration...

To maintain cell viability, all injections were performed within one hour. So they are busy with one model mouse, and then move on to the next one.

"Adjust the cell concentration to 1x10^7ml..."

"Yuhan, you are responsible for taking 0.2ml of cells per injection."

"Team leader, the anesthetic is ready!"

Each of them had high fighting spirit, and no one cared about what happened to the local tyrant Jun, and even forgot that he was standing there watching.

Gu Jun watched quietly, watching He Yuhan prepare the cell suspension, Cai Zixuan anesthetized healthy mice with an intraperitoneal injection of sodium pentobarbital, Xu Hai and Zhang Haoran then marked the mice and depilated their buttocks, and finally Wang Ruoxiang gave them Tumor cells were injected subcutaneously in the right buttocks of mice...

No. 1, No. 2, No. 3, one after another.

Suddenly, Gu Jun's head burst into pain. Could it be a brain stem tumor...

The pain suddenly intensified, and he couldn't help crying out, "Ah!" The scene in front of him became dazed, flickering like the shadow of a nightmare.

He seems to be out of breath, as if he is in a dilapidated laboratory, with centrifuges, microscopes, sterilizers and other instruments lying on the ground, broken test tubes, messy documents, blurred picture frames, rancid animals The corpse... The dust turned it all into a gray color, without a breath of life, only chaos, dead silence.

The pain in the brain became more and more serious, but the scene became clearer, and he saw some indescribable instruments.

And those twisted corpses on the ground... I can't identify what animal it is...

He saw again that a line of blood was painted on the wall inside, and it was the mysterious language on the medicine box, but compared with the standard of printing, the strokes of the blood had a terrifying kind of stroke. weird.

In a trance, Gu Jun suddenly seemed to be able to understand the meaning of the bloody words.

He muttered softly.

Love the plague doctor.