Plague Doctor

Zhang Lin took out the lifting tube from the liquid nitrogen tank, then took out a cryovial from the lifting tube, and put the lifting tube back carefully, because there were still many cryogenic tubes of other cells in the lifting tube. After Zhang Lin sealed the lid of the liquid nitrogen tank, he began a series of patient and meticulous operations.

He first put the cryopreservation tube in a water bath, thawed it quickly with 37c hot water, then changed the centrifuge tube and centrifuged it for five minutes, then sat down on the ultra-clean workbench, and baked the centrifuge tube nozzle to the alcohol lamp After baking and sterilizing, discard the waste liquid, add fresh culture medium with a pipette, and mix by repeated pipetting...

Gu Jun has been watching carefully, and if he has any doubts, he will ask Senior Brother Zhang for advice, and he has learned a lot.

Like the rotation speed control of the centrifuge, the suction and release control of the pipette, etc., any sloppy recovery operations will affect the survival rate and proliferation rate of cells.

Finally, Zhang Lin injected the cell suspension into the petri dish, added an appropriate amount of culture medium, and put it into a 37c, 5co2 cell incubator.

"Wait, it will take at least two days." Zhang Lin took off his mask and pushed on his glasses, "I suggest you keep it for a week, so that the tumor formation rate can be guaranteed."

"Let's try a batch after raising for two days." Gu Jun shrugged and said, there is no way, there is not enough time, the system shows that his condition will worsen after 30 days, if it is true, it may be difficult to get out of bed and walk by then. He said again: "Senior brother, go out for a meal at night? I'll treat you."

"No." Zhang Lin waved his hand, "I'm going to give medicine to the mice at night, I'm a treat."

...

For the next two days, Gu Jun spent the next two days on campus. In addition to gathering with friends, firstly, reading books to review and preparing for make-up exams. It would be quite troublesome to repeat the grades if he could not die; The experimental building takes care of the cells and changes the culture medium once.

On the morning of the third day, Zhang Lin personally came to see it and said that the cells were barely growing. He helped with subculture and preparation of cell suspensions.

And Gu Jun went to the school's experimental animal center. This is actually a large breeding base. It is located a few hundred meters outside the school campus. It is very lonely, probably because it is filled with the stench of various animals.

He bought five nude mice, each of which is more than 100 yuan, which is quite expensive. Fortunately, he still has 20,000 yuan in savings, but it will not last long.

When the money is used up, I hate it. Gu Jun really regrets donating the money so early... People are still alive, and the money is gone.

cough cough. Each of these nude mice is about the size of two or three fingers together, and they have no hair. They are kept in a sterile box. Once they come into contact with the outside environment with bacteria, they will easily become infected and become sick and die. Therefore, the usual feeding and experiments need to be carried out in a barrier environment.

The barrier environment laboratory of Dongda University is located in the Experimental Animal Research Center. This five-story yellow brick building stands next to the experimental building.

Gu Jun and Zhang Lin met in the lobby on the first floor of the research center and booked a one-hour use of a small nude mouse room. This is a small room with only 21 square meters, which is divided into a front room and a back room. The front room has laboratory benches and instruments, and the back room is a breeding room.

Before entering, they and all the items they bring must undergo a strict ultraviolet sterilization and disinfection. The two set foot in the nude mouse room with a special full-body protective suit, masks, gloves, foot covers and other full equipment.

Soon, on the laboratory bench in the front room, Gu Jun marked five nude mice by punching, and then injected tumor cells subcutaneously on the right side of their buttocks one by one.

Zhang Lin was in charge of assisting. These cells could theoretically form tumors when they hit people. Now that the local tyrant Jun is so enthusiastic about the nude mice, he is sure that this little brother is not trying to play murder.

After all the five nude mice were injected, Gu Jun put them in the rearing cage, and then saw whether they could form tumors.

"Senior brother, thank you very much." Gu Jun gave Brother Zhang a thumbs up. Without his help, it would not have been so smooth.

"The old Chinese doctor you mentioned..." Zhang Lin still had Conan's vigilant look on his face, "Isn't he really a liar?"

...

For the next week, Gu Jun continued his previous life rhythm, except that he went to the nude mouse room every day to see the cage of nude mice. Their feeding is uniformly taken care of by the management staff of the nude mouse room, and each animal costs 3 yuan per day for board and lodging.

This week, he only completed three ordinary tasks, because those who need to treat and save people can't do it. Although the hospital is next to him, he is not a doctor yet. The tasks he completed were all related to medical practice. There were already 5 boxes of pills in his hand, and the proficiency of his hands reached the first level (30005000).

On the seventh day, Gu Jun walked into the nude mouse room again in sterile equipment and observed the nude mice one by one.

None of them died. A bean-sized round tumor had grown under the skin on the right side of the buttocks of No. 1, with a diameter of 0.5cm after measurement; No. 3 and No. 4 also had obvious protrusions, with diameters of 0.3cm and 0.5cm; No. 2 and No. 5 still have no signs of tumor formation.

So far, he can be sure that three of the five nude mice developed tumors.

Gu Jun set two tumor-bearing nude mice as group a, one tumor-bearing nude mouse as group b, and two non-tumorous nude mice as group c and group d, and went to the animal center to buy 10 healthy nude mice. , 5 only set e group, 5 only set f group. Groups a, c and e will all receive the same dose of "exotic medicine" (his name) every day; while groups b, d and f will not receive any medication, only for control purposes.

That is, tumor-forming nude mice, non-tumor-forming nude mice and healthy nude mice each have one group with medication and one group without medication.

At this time, six cages had been allocated on the experimental bench, and the nude mice were chirping and screaming.

Gu Jun fiddled with 25 Yiwen medicine capsules. Since the system stated that one box can prolong life by 5 days, and a box of 5 capsules, it should be one capsule a day. This is the adult human dose, and the average weight of these nude mice is less than 20g. If they also take one pill a day, it is estimated that they will be sacrificed immediately.

He poured out all the powder in a capsule and weighed the dose, then converted the dose of nude mice based on his own weight, made a solution, and diluted it proportionally.

Soon, Gu Jun held the gavage needle filled with potion in one hand and a fixed healthy nude mouse in the other hand, and started the first gavage administration.

"Hawhaw." The nude mouse struggled a little, but this poor thing was still pushed by him with this potion to make its contribution to medicine.

At this moment, Gu Jun's heartbeat was a little faster, and he stared at the nude mouse in his hand without blinking.

He hadn't done any toxicity tests on exotic medicines until this moment. If it died suddenly, perhaps the experiment would be meaningless. And now, he stared at it for five minutes, then 10 minutes...

Half an hour later, the nude mouse still did not show any abnormality.

"It seems that Yiwen Medicine has no obvious acute toxicity." Gu Jun breathed a sigh of relief, "Chronic toxicity needs to be observed."

Only then did he continue the injection of the next nude mouse. It took ten minutes to complete the administration of the other seven nude mice in the three administration groups, and he observed them for another hour. All of them had no abnormal reaction.

"All rat friends, live." Gu Jun muttered, pressing his aching head, his condition was obviously getting worse day by day, "Please."

...

On the second and third days, Gu Jun continued to work in the nude mouse room every day.

The tumor of the nude mice in group b without drug has increased from 0.3 cm to 0.6 cm, and the body weight and mental state have not changed; while the two nude mice in group a with drug treatment, after two days, the tumor size is the same. No change, maintained at 0.5cm.

Gu Jun knew what this might mean, the different medicines are really effective! These capsules can significantly inhibit tumor growth.

In addition, the other nude mice were administered as usual, which also means that the different medicines did not seem to have adverse reactions and did little damage to body functions.

"Maybe, this time it's really saved?" He thought to himself. Although the unease still lingered in his heart, a new hope burned at the same time.

The fourth day, the fifth day... in a blink of an eye, a week has passed.

The tumor of the nude mouse in group b has grown to 1.5cm. The huge tumor is in sharp contrast with its thin body. It began to lose weight and became less mental. However, the two nude mice in group a continued to be in stable condition, and all the nude mice were alive and well, which was no different from the nude mice in the blank control group.

I have to say that the results of this experiment are very satisfying.

The efficacy of different medicines is better than that of the top tumor small molecule targeted drugs currently on the market.

However, Gu Jun also knew that the adverse reactions of the different medicines could not be completely ruled out, which just proved that it had no side effects in a short period of time. And this is an animal experiment after all, which is different from a human clinical experiment.

However, this experimental result is already worth his risk.

This afternoon, after writing his suicide note, Gu Jun came to the waiting area of ​​the emergency department of the Affiliated Hospital of Dongda University and sat down on a rare vacant waiting bench. The surroundings were crowded with patients and their families, mostly crying children and comforting parents, nurses hurried around, nurses pushing rescue beds...

There was a lot of noise, and the emergency department broadcast continued to call: "No. 182, Chen Ziyang. No. 183, Wang Yuxuan. No. 184, Huang Zixuan..."

Taking a breath, Gu Jun resolutely threw a Yiwen medicine capsule into his mouth, took a sip of mineral water and swallowed it.

"Come on." He looked calm and looked at the emergency sign on the wall over there, "If there is any toxic reaction, come on."

Love the plague doctor.