Rebirth of the God-level Xueba

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Yang Rui looked at Gu Qiang's wild laughter inexplicably.

A normal person, under such gaze, would be unable to laugh out of embarrassment for at most ten seconds.

However, who is Gu Qiang? He's a man with a poking Teddy spirit.

Under the eyes of everyone and even dogs, Teddy can grab a dilapidated doll and rape it happily; under the eyes of everyone and even dogs, Teddy can grab a shopping thigh and move it lively and lovely; Teddy can move slowly in the air frequently and efficiently under the eyes of everyone and even the dogs; Comrade Gu Qiang can naturally laugh heartily and freely under the eyes of everyone and even the (research) dogs extremely.

Yang Rui couldn't help but sighed deeply.

Everyone says Teddy is good, that's when you haven't got white slime on your legs, and everyone says technicians have personalities, that's when your ears don't hear their manic laughter.

"Comrade Gu Qiang, lower your voice, don't affect other people's experiments." Yang Rui waited for Gu Qiang to laugh, and said: "We are doing repeated experiments on frozen embryos, which is nothing innovative. Don't get excited."

"Haha..." Listening to what Yang Rui said, Gu Qiang couldn't help laughing again, and said, "If I have a tape recorder, I'll record it and play it for the Chinese Academy of Sciences to listen to!"

Gu Qiang coughed, cleared his throat, imitated Yang Rui's voice, and said: "We are doing repeated experiments on frozen embryos, which is nothing innovative."

Gu Qiang laughed wildly again, and said: "The people of the Chinese Academy of Sciences are going to be mad, hahahaha..."

I have to say that Gu Qiang's parrot is really cheap.

Yang Rui sighed again, working with such a person is indeed troublesome.

This time, Yang Rui felt that it was not good to mention the name of the person in charge of the laboratory. He stood up a little straighter and said, "Researcher Gu, you may not know that there are always laughing people outside the window of our ion channel laboratory. sound."

"Huh?" Gu Qiang didn't understand.

"When the ion channel laboratory was not built, I led an experimental group to work on the potassium ion channel project. At that time, the laughter was even louder. After all, the research on potassium ion channels at that time was a worldwide innovation." Yang Rui glanced Looking at Gu Qiang, he said slowly: "No one thought that I, who was a student at the time, could lead a team to produce a world-class basic biology project. Inside Peking University, Professor Richard from Berkeley also supported me..."

The researchers in the laboratory may not have experienced that scene, but thinking back to those days, as a member of the ion channel laboratory, they still puffed up their chests unconsciously.

Yang Rui looked at Gu Qiang with a smile, moved his lips, and said, "Afterwards, when I did PCR, no one liked it, and my smile was spread abroad, and I even filed a lawsuit for it. In the end, I still kicked those smiling faces."

"As for the drug development of deferiprone, you may not know about it." Yang Rui looked at Gu Qiang with a disdainful expression unabashedly, and said: "But those who were embarrassed to laugh back then are embarrassed to show up in front of me now." gone."

Yang Rui paused for a moment, turned to Gu Qiang, and asked, "Now, I say I'm going to do a frozen embryo experiment, who dares to laugh?"

The researchers in the laboratory couldn't help looking at Gu Qiang.

The smile on Gu Qiang's face had long since disappeared.

He seems to recall only now that Yang Rui is a scholar who holds articles in the three major journals of S.

In China, there are naturally some scholars who can do this.

However, anyone who can do this is not the top scholar.

Such a scholar, who would dare to laugh at repeating foreign experiments?

Gu Qiang suddenly felt that he seemed to be laughing a little too early.

"Genetic engineering is still different from basic biology." Gu Qiang tried his best to find a similar explanation.

Mainly face to face,

Gu Qiang couldn't believe that Yang Rui, who was so young in front of him, could make so many achievements.

Furthermore, as he said, Yang Rui has never done genetic engineering after all, so why repeat the experiment?

Repeated experiments are different. For some old experiments, such as those done in middle school laboratories, all the details of the steps are very clear, and there are professional analysis on where the mistakes will go. Just pull a student out , As long as you know the characters, understand the meaning on paper, and spend a little time, you can do those experiments.

However, back then, some experiments in middle school laboratories were also kept secret.

It is true that there will be papers in journals, and there will be experimental procedures in the papers. However, if there is no detailed explanation, if you want to repeat these experiments, you will always have to spend some research time.

This is the case for more complex experiments.

Like the transfer of frozen embryos, there are too many details, and if you don't pay attention, you will make mistakes.

Live embryos need to be frozen and resuscitated, then transplanted into living organisms, and then conceived to give birth to cubs. If you make a mistake, you may lose everything. Moreover, because the process is too long and takes too long, the cost of trial and error is quite high of.

If this is not the case, how can domestic laboratories be willing to give up this opportunity to "fill the gap in the country".

Yang Rui's understanding is consistent with Gu Qiang's. Genetic engineering and basic biology are indeed different, but for him, the difficulty of repeating experiments is completely different.

In 1985, of course, foreign institutions were extremely defensive and refused to disclose the details of frozen embryo transfer.

Because although they have completed the frozen embryo transfers of mice, cows, and sheep, they have not done the frozen embryo transfers of pigs, dogs, and monkeys—the frozen embryo transfers of the first few important animals are proper S-level papers , How can it be easily handed over to competitors.

However, after the mid-1990s, this information was of little value.

Even if Yang Rui checked the public information, he could find countless materials.

In particular, some master's and doctoral dissertations, due to their length, are mostly repetitive experiments, and the content is really detailed. It's a hundred-page paper, and if you don't write detailed experimental details, do you still want to discuss industry gossip?

Even if some papers have problems such as inaccurate data, it is still possible to find specific details by referring to each other.

The corner of Yang Rui's mouth twitched, but he stopped playing with Gu Qiang and said, "Let's start the experiment."

The problem that needs to be solved is solved, and he doesn't need Gu Qiang to be convinced.

Gu Qiang obediently stood behind the experimental table and started a new preparation.

"Have you ever done an experiment on frozen embryos?" Yang Rui took the outline he had compiled and revisited the various steps in his mind.

Gu Qiang said: "I have read the information, but I have not operated it in the field. The equipment is too expensive."

He looked around and mumbled a little unconvinced.

The Ion Channel Laboratory is for basic biology, and it is beyond the envy of ordinary scientific research institutions to be able to cobble together complete genetic engineering equipment.

"I haven't done it well. I have several experimental designs here. Let's do a cross-comparison." Yang Rui said and handed a piece of paper to Gu Qiang.

"Ken took the experimental design?" Gu Qiang snorted twice, picked it up and looked at it, and said while watching: "Put the embryos into the balance liquid to balance, install the tubes, cool down, plant ice, and rebalance... Hehe, this kind I have read the article a long time ago, how many degrees does the temperature drop to? What is the balance liquid used for? Without these materials, how can I do it?"

The question he asked, apart from his tone, was really unrealistic.

Yang Rui was not surprised and said: "Let's talk about the balance liquid first. We use glycerin."

"Glycerin? Glycerin can regulate cell dehydration and protect protein structure. However, glycerol is only suitable for slow freezing."

"We also don't have equipment for rapid freezing."

"What about the concentration?"

"Temporarily select 2% moles per liter, and use buffered pb2 to 7.4..." What Yang Rui said is all about the details, such as the pH value, its fluctuation range, in ordinary experiments I don't care, just look at the ph of the final result. But here, it doesn’t work. This requires that during the configuration process, we must always pay attention to ph, and adjust the order and rate of filling. If there is no relevant information, it may take ten years of trial and error.

Gu Qiang gave Yang Rui a strange look, but muttered, "People are more angry than others."

However, he thought that Yang Rui got this information from a certain foreign scholar. The so-called academic contacts are sometimes very unfair unspoken rules. Gu Qiang does not know a foreign scholar. Opportunities showed that although Yang Rui did not know how to operate genetic experiments, he was able to obtain relevant information, but he was firmly seated as the project leader.

There is nothing wrong with the preparation of the balance liquid itself, so Gu Qiang stopped talking and concentrated on the operation.

After a while, Xu Zhengping came over and said, "Director Yang, the list of the third batch of visitors has been finalized. You can see how to arrange it. The main thing is the time for visiting the laboratory."

"Let them come to visit the frozen embryo transfer project." Yang Rui answered neatly.

Xu Zhengping was taken aback for a moment. High-tech projects are naturally good, but they must have results to be interesting.

Gu Qiang said without turning his head: "I can't promise to make it."

"It's fine as long as you don't make mistakes in your technique." Yang Rui replied even more irritatingly.

Xu Zhengping was a little uneasy, but he still nodded, approached Yang Rui again, and whispered, "This time there are Professor Zhang Feng from Nannong, and Professor Ouyang Shi from Shanda University."

"Two big cows?"

"Yes."

"I'll tell Professor Cai." Yang Rui felt that it was still difficult for him to eat two big cows in one go, so he immediately thought of Professor Cai who had a good appetite. He had promised this before.

Gu Qiang frowned when he heard the word Shanda, but his movements became more careful.

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