Rebirth of the God-level Xueba

"PCR is indeed a technology that can win the Nobel Prize. It is so easy to use." Ouyang Shi stood in front of the PCR instrument, like a very hungry person standing in front of a barbecue grill, with a gentle face.

Yang Rui stood in front of him with a grin, watching Ouyang Shi operate.

He was injured from playing PCR in his previous life, so he wouldn't do it if he could.

Gu Qiang didn't have Ouyang Shi's deep emotion. Across the experimental table, he operated the microsurgical knife while laughing twice, and said, "The one you made is not technically difficult at all. It's boring, Xiao Liu, take it." Walk."

He divided another group of embryos and called his assistant to send them away.

Xiao Liutong is a researcher in the laboratory, but he is not good enough, so he should be an assistant as well as an assistant.

Of course, there are also advantages to being an assistant. He can learn some operating methods of the microsurgical knife from Gu Qiang. As far as this technique is concerned, if he learns 80% of Gu Qiang's skills, he will have no worries about eating and drinking for the rest of his life—that is, in the 21st century. There are not many biotechnology service companies that can master this technology, and few are willing to use expensive bovine embryos to train you. Everyone hires skilled workers with high salaries, and no one is trained.

The embryos divided by Gu Qiang will be sent directly to another group for the steps of freezing and thawing embryos. Although it is still in the scope of experiments, it is also a step of practice, otherwise they will not be frozen one by one.

Compared with the embryo segmentation done by Gu Qiang, the operation of frozen embryos is much less difficult, mainly due to the configuration of reagents and the ratio of addition. The latter is part of their main training, while the former is Yang Rui's job.

In order to receive money from the technical service company smoothly in the future, Yang Rui deliberately concealed some key details, such as the configuration of the reagents, which he will complete the whole process. Others do not have the formula, and they cannot repeat the experiment even if they know other steps.

Compared with Gu Qiang dividing embryos one by one, the workload of reagent preparation is much lower. Yang Rui can prepare a large amount of solution at one time for long-term use, with low cost and short time.

It took only a few days for the entire genetic engineering laboratory to enter the smooth mode, which was also due to Yang Rui's experience.

Up to now, he has managed the ion channel laboratory and Huarui laboratory respectively for two years, and the management experience he has accumulated is really not inferior to other domestic managers.

Today's scholars who know the laboratory may not necessarily have as much experience as him.

Of course, Yang Rui's reputation is also very important.

For example, Comrade Ouyang Shi was once known for his rebelliousness. When he was at Shanda University, he was even more aggressive than Gu Qiang, but in the genetic engineering laboratory, he was willing to accept Yang Rui's trend.

Hearing that Gu Qiang said that PCR technology is not difficult, he was not only not angry, he was also very happy and said: "Good technology, mature technology, it is easy to do, otherwise, how to promote the technology?"

"The technique of freehand segmentation is simple, but Director Yang won't allow me to use it." Gu Qiang still couldn't forget this. Compared with operating a microscopic scalpel, the technique of freehand segmentation is more sophisticated, but it is more comfortable.

Yang Rui had nothing to do under his command. He watched the two of them work, and said calmly: "There is no potential for freehand segmentation. The plan of the microscalpel is well done, and the success rate has increased so fast."

"How can there be no potential to tap, I think I can improve a little bit." Gu Qiang said a little arrogantly, his experimental level, not to mention learning from others alone, but it is no problem to be alone in a few laboratories.

Yang Rui chuckled twice.

Ouyang Shi was impolite, and immediately commented: "Boasting."

"You don't believe it? Let's try it?" Gu Qiang looked like he was about to put down the microsurgical knife immediately.

"If the embryo is damaged, the funds will be deducted." Ouyang Shi chuckled and grabbed Gu Qiang's seven inches.

Gu Qiang lowered his head helplessly, and could only operate while operating, saying: "I'm doing two experiments at the same time, and I can do it too."

"So what if you can do 10 experiments at the same time? If you can come up with a technique like PCR,

This is the real one. Ouyang Shi gave a thumbs up, and said again: "Do you know why PCR is eligible for the Nobel Prize?" "

"Oh?"

"Because pcr has promoted the development of biology and human beings." Ouyang Shi seemed to be reading a textbook, but he was very pious on the outside. He admired Yang Rui and even became an "uncle fan" because he started from pcr.

There are too many new technologies developed on the basis of pcr technology.

For example, the detection of x-chromosome-associated enzymes and y-chromosome-specific DNA probes, if calculated for their social benefits, are at least at the level of tens of billions of yuan. If you don't limit your expenses, you can support at least 10 tattooed girls who smoke, drink, speak dirty words but know they are good girls.

As far as the derivative value of the entire pcr technology is concerned, it is no problem to feed several women in the group army.

It can be said that pcr technology has opened up a new chapter in biology, just like the microscope opened the door to the microscopic world, pcr technology opened the door to the dna era.

However, in the genetic engineering laboratory, Ouyang Shi is the only one with an international perspective.

Ouyang Shi has not only participated in international conferences many times, but he can also understand and publish English literature. This ability is common among middle and high-end scholars at Peking University. However, if you look at it nationwide, But it is very rare.

Therefore, in the whole laboratory, the most excited person is always Ouyang Shi.

He knew how powerful Yang Rui was.

For example, pcr technology, most of the Chinese researchers at the technician level don't know what it is, and a small number of technicians with some level can't really appreciate the coldness of it.

Whether it is Gu Qiang from Shanda University or researchers from other universities, although they know that their work in the genetic engineering laboratory in Haidian District is very good, they are still stuck in the idea of ​​"filling in the gaps". Yang Rui is imitating foreign technology, but cannot appreciate the profound changes in technology.

Now there are no online forums, no instant messaging software, and even no mailboxes. Everyone's information sources and insights are limited to those around them, and there is nothing they can do about it.

At this time, Yang Rui didn't have time to talk about PCR's contribution, so he waved his hand and said, "Don't brag, let's do the work first, and if the work is done, the grades will come naturally. Professor Ouyang, your side is cooked."

"Oh, I said I forgot." Ouyang Shi said as he took out the box of the PCR instrument.

Today's pcr instrument is the first version improved by Yang Rui, which is much simpler than the original pcr, with automatic power-off protection and so on. It is basically equivalent to simplifying the work of cooking spicy hot pot into The rice cooker cooks rice.

Ouyang Shi paid a little attention and made two records, which means he completed a set of experiments and made records.

Gu Qiang was even more dismissive of this.

Yang Rui ignored him at this time, and turned his attention to Ouyang Shi's experimental table.

The sex identification of embryos is the last big hurdle they face, and it is a big hurdle they have not touched before.

Regardless of whether he was doing mouse or rabbit embryo transfer, Yang Rui did not arrange sex identification. After all, not doing sex identification would not affect the operation of the entire system.

However, when it comes to commercial bovine embryo transfer, it is impossible not to break through the barriers of sex identification.

When doing experiments, no one cares about how many male and female mice you transplanted from the embryos, but if China Animal Husbandry spends money on embryo transfers of 1,000 cows and ends up with four or five hundred bulls, it will be crazy. What's the use of having a bunch of bulls? Even if they want to slaughter them and sell them abroad, the number of 400 to 500 is not enough for channel construction. As for breeding, it has been 85 years now. Of course, the precious Simmental cattle must be artificially inseminated. A strong male It is easy to breed millions.

Therefore, as far as the entire commercial embryo transfer system is concerned, embryo segmentation, frozen transfer, and sex identification are equally important. Of course, techniques such as superovulation are also very important, but because they are not difficult, they are not considered by Yang Rui at all.

The main obstacle to gender identification is the high requirements for early identification.

In fact, identifying the sex of 8-cell embryos is only the minimum requirement for the commercialization of sex identification. For up to 10 years, the minimum requirement is only 2 cells to identify the sex of cattle embryos by using the y-chromosome-specific DNA sequence.

That is to say, once the fertilized egg has doubled, sex identification can be done.

This method naturally reduces the cost of commercialized embryo transfer again, because it is a method of artificial insemination, the fertilized eggs are definite, and the time for doubling into 2 cells is also definite. In this way, one round After the identification, embryos that do not meet the requirements can be quickly removed and repeated with the least loss, thus greatly improving the efficiency of commercial embryo transfer.

If it is a large-scale biotechnology service company, it can also directly take out the embryos and distribute them according to the requirements of different customers, so that Modern Dairy is under the control of industrialization from the beginning to the end.

The acquisition of this technical advantage makes sex identification an important technology that is not inferior to embryo segmentation and cryopreservation.

However, this technology is only just beginning to be developed around the world.

No way, Yang Rui only came up with pcr technology for two years, and before the invention of pcr technology, whether it was cytogenetic analysis or the difference analysis of embryonic development rate, the accuracy rate was relatively low, and the cost was relatively high, and there was no Not popular with closets.

Correspondingly, after the emergence of pcr technology, sex identification before embryo transfer became very popular, not only by the shepherds, but also by bio companies. Correspondingly, sex identification before embryo transfer became Trade secrets of competition among biological companies.

Techniques such as embryo segmentation and cryotransplantation were developed around the 1970s. By 1985, even if the patent period had not expired, they were widely used.

Gender identification is different. As the latest technology, pcr has promoted the research and development of a variety of new technologies, and these new technologies, no matter whether they are patented or not, are of course kept secret.

As a result, Yang Rui naturally could not use foreign materials to explain his directional guidance, but had to overcome this technical difficulty step by step.

Original research is basically equivalent to complicated research.

"Just treat it as a trump card." Yang Rui comforted himself in this way, and then devoted himself to Han Peidong's information and data.

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