Rebirth of the God-level Xueba

"Please first." Director Mei gave way, but felt that it was inappropriate, so he couldn't help adding, "Director Yuan, Yang Rui didn't know that you did scientific research, this"

"I understand, I understand." Director Yuan smiled with the majesty of a superior, and said: "I just watch but don't talk, let's go back, and I will nag you again."

"You're joking. When the time comes, I'll put on a table and invite experts to come and listen to your explanation and analysis." Director Mei felt a little relieved when he saw that Director Yuan finally knew what he was asking him to do.

He wanted to understand the situation in the laboratory, but he didn't want to offend Yang Rui.

In an instant, the two registered at the guard and entered the laboratory.

Director Yuan looked around, still looking disgusted, and said, "This laboratory is just an old factory building, right?"

"It used to be a warehouse." Director Mei replied in a low voice.

Director Yuan dragged, and said: "This is not acceptable. Foreign laboratories, not to mention the advanced equipment inside, must first improve the infrastructure. I think the paint on the top has fallen off. Don't let the paint fall off. Affect the experiment."

Director Mei smiled and said, "No."

"Why not, those who do experiments, you must pay attention to these."

"I reckon, there should still be no money."

"Didn't you donate money to them? Where did the money go?"

Director Mei was a little surprised and said, "You know about this?"

"Who doesn't know." Director Yuan smiled and said, "China Animal Husbandry Corporation donated millions of dollars in one go, and everyone in the circle is watching."

Just saying this made Director Mei a little nervous.

He looked around, and couldn't help but feel that the infrastructure was too old. Thinking about what Yang Rui said before, Director Mei still explained on his behalf: "It is said that many steps have to be taken in the embryo transfer of this cow. The farm's The environment is worse than here, so there is no need to spend a lot of money to renovate the laboratory."

"What you said is correct." Director Yuan pondered for a while, nodded slightly, but still looked disgusted, and said: "Actually, in the final analysis, it is just one word, poor. Our domestic conditions are poor and funds are scarce. This is an objective condition, an objective condition Under the constraints of the environment, it is not easy to do research, so instead of doing embryo transfer in foreign laboratories, it is better to do some simple research, don’t you think?”

The first half of Director Yuan's words were pleasing to the ear, but the second half made Director Mei a little reluctant.

However, the two had already entered the laboratory, so he couldn't quarrel with Director Yuan right now, so he could only nod his head depressedly, and said, "Here we are, the one on the right in front is Yang Rui."

"Pretty young."

"Yes, I'm only 22 years old this year."

"I'm only 22 years old, and I already have a laboratory." Director Yuan clicked his tongue twice and said, "When I was 22 years old, I was still teaching there. I searched all over the county, but I couldn't find a bottle of concentrated sulfuric acid."

Director Mei smiled and nodded again, then greeted the back in front of him: "Director Yang, I'm here again."

"Yo, Director Mei." Yang Rui looked back, saw Director Yuan, and asked with a smile, "Who is this?"

"Director Yuan is the leader of our Science and Industry Committee. I talked about it yesterday. Today I came to visit our laboratory." Director Mei tried to put it lightly.

Yang Rui smiled in surprise, "This is the leader's inspection."

After saying a few nice words, Yang Rui did not take the lead in the inspection after all. After all, he has no affiliation with the Science and Technology Committee.

"Director Mei, Director Yuan will leave it to you to entertain." Yang Rui didn't have a deputy like Xu Zhengping in the genetic engineering laboratory, so he simply threw it back to Director Mei.

"No, I'll just take a look at the side and learn about your work." Director Yuan came here to watch Yang Rui sit in the experiment. He found a chair and sat down, and began to observe.

Yang Rui was a little puzzled, but he continued to work as usual.

He has already done the part of providing primers for pcr. This work cannot be learned just by looking at it with the naked eye.

In fact, most of the experiments cannot be learned by looking at them, not to mention those complex chemicals that make people confused about the types, contents and ratios. Most of the time, primer design is based on luck.

The so-called primers are the primers for the PCR reaction.

In order for PCR to work, primers must first be used to seduce the required DNA fragments.

Therefore, primer design is a key step in the successful amplification of PCR. Good primers can not only avoid the generation of background and specific substances, but also recognize genomic templates and so on.

However, primers are artificial, just like drug primers, which are always hard to come by.

In the 21st century, primer design is a relatively simple task. There are not only general genes that can be used for reference, but also a large number of design templates, and there are even three huge international nucleic acid databases for querying corresponding information resources.

Of course, corresponding primer design software is also available.

Even so, the vast majority of scholars will not design primers by themselves, because this work is cumbersome and professional, and they would rather find a professional company to outsource this work.

Only when people are at the forefront of the industry and are unwilling to reveal the breakthrough points and focus points they are exploring, will someone design their own primers. Of course, there are those who can't afford it, and old-school scholars will do the same.

In 1985, Yang Rui naturally couldn't find any usable primers.

In fact, he could hardly find anything, not to mention primer design software or the corresponding nucleic acid database, even the simplest general template, he couldn't even think of getting one.

The current pcr research is in full swing of the competition - naturally not the competition for pcr itself, but the scope of the use of pcr.

It's like when X-ray first appeared, a large number of scientists started from their own research fields and thought of a way to use it, so everyone rushed to do it, at least, they rushed to make the simple part first. .

There is no easier way to brush your face, reputation, and impact factor than this.

In this state of intense competition, you cannot expect well-meaning academics to publish their hard-earned primer templates. Even if there is, it is usually an incomplete part.

Fortunately, Yang Rui himself had designed the primers, and with the support of various documents, he was qualified to do it.

Of course, during the period of designing the PCR, Yang Rui also had to make some primer templates, but they couldn't be used on the topic of gender identification.

He even had to start with the genetic sequence of the cow.

This is actually embedded in the genome project again. The goal of the Human Genome Project is to sequence every gene in the human genome and mark it like a map. Part of the function lies in the convenience of running PCR.

And in '85, there was neither a human genome project nor a cattle genome project.

Any unit or individual who wants to know which segment of the gene should sequence it by themselves.

In fact, this is not particularly difficult. The human genome is composed of 3 billion base pairs, so it took a lot of time, manpower and money. The core of a specific gene of the sry sequence that Yang Rui needs The sequence, even if it is based on the requirements of the 1980s, only costs tens of thousands of yuan.

Of course, there are very few laboratories that can do gene sequencing. In China, there is Yang Rui's ion channel laboratory.

The ion channel laboratory established a special research group a long time ago to do a lot of gene-related research, and it is not very difficult for them to do a special gene sequencing independently.

If there is a request from other laboratories, it is natural to discuss and arrange the time. Yang Rui has a request, as long as the corresponding funds of the genetic engineering laboratory are allocated to the ion channel laboratory, the results will be easily obtained.

It was also on this basis that Yang Rui started the design of the primers.

I saw him doing paper work most of the time, and dealing with bottles and cans for a small part of the time.

Director Mei was dizzy and dizzy from watching, and Director Yuan was also sweating from the top of his head.

Director Yuan knows the PCR instrument and the principle of PCR. He has also used the PCR instrument, but that is all.

Director Yuan came back from England more than a year ago. At that time, PCR was made by Yang Rui. Therefore, Director Yuan did not come into contact with PCR and its use abroad.

Back in China, Director Yuan has not been working in the front line for a long time. Although he has the tradition of reading journals and often going to the front line, he really doesn't know much about the emerging PCR.

Of course, if Yang Rui uses PCR normally, it will not be difficult for Director Yuan. It is nothing more than an instrument that is more troublesome than an X-ray machine. For scientific researchers, it is no more difficult to operate than a rice cooker.

However, when Yang Rui started to design the primer, Director Yuan was blinded.

Most scholars who have a general understanding are going to be caught blind.

Under Director Mei's expectant eyes, Director Yuan was even more embarrassed.

"Let's go back." At noon, Director Yuan couldn't wait to walk out of the laboratory.

Director Mei followed quickly, and asked in a low voice, "How do you look? Is their progress reaching the point where they are about to produce results?"

Director Yuan remained silent, looking like he was thinking.

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