Rebirth of the God-level Xueba

Making primers is a very difficult job, perhaps not as difficult as "using seven to forty-nine virgin boys and girls as medicine primers", but it is also more difficult than rootless water from the desert.

In the era after the vigorous development of computer technology, biologists specially developed special software for making primers, such as the powerful and easy-to-use pre, common, or vectorntisuit, dnasisomiga, dnastar, etc., just look at the number of software You can imagine how complicated this is. As for the version number, it can better explain that these software have not solved all the problems.

In fact, even though pcr has been invented for 30 years, designing primers is still not a routine science. Of course, scholars have summed up some laws, but uncertainty and chance still plague scholars.

Yang Rui did not require working hours in the early days of the establishment of the Genetic Engineering Laboratory, and now he is offering a magic weapon of 110 hours again because of this uncertainty and chance.

Uncertainty and contingency can be summed up as probability, and the most natural way to solve probability problems is to increase samples.

The researchers in the entire genetic engineering laboratory have dedicated themselves to completing the primer design work.

Publishing papers in first-class Chinese journals is Yang Rui's reward for them. Correspondingly, hard work is inevitable.

On this point, Yang Rui is very frank.

The gap between Chinese laboratories and laboratories in developed countries is huge, from funding to instruments, from management to technology, from environment to accumulation - the reason why they can still produce world-class results, the only thing that can fill these huge gaps, It is the blood and tears of experimental dogs and scientific researchers.

Modern laboratories all have access control systems. Whether it is punching in, fingerprinting, or iris that is not good for cattle, it can record the working hours of experimental dogs and scientific researchers. Comparing the attendance time of the laboratory, the gap can be clearly seen.

Most foreign laboratories work on an eight-hour working day. The more developed countries are, the more fixed the working hours are. But if you look at domestic laboratories——Tsinghua’s biological laboratory, the monthly attendance of doctoral students The time is about 450 hours.

450 hours of attendance per month is much harder than 110 hours of attendance per week, which means that Tsinghua doctoral students will not rest for more than 3 days a month, and it is impossible to sleep for 8 hours a day. And, except for eating and sleeping, the experimental dogs must dedicate all their time to the laboratory.

If science needs sacrifices, its sacrifices are the time, blood, tears and hardships of laboratory dogs and scientific researchers.

A large part of the reason why Yang Rui did not continue his studies after completing his master's degree was that he could bear the passing of time and blood and tears, but it was difficult for him to endure the hardships of studying.

Doctoral students with an average age of 25 and above are already standard adults. However, they can only earn about 1,200 yuan for 450 hours of labor per month. It is not an exaggeration to say that it is difficult.

The former Yang Rui struggled hard, but still couldn’t get out of the quagmire of real life—when you returned to the dormitory after a hard day, exhausted, hungry and thirsty, picked up a box of lunch meat, but couldn’t bear to open it, schoolwork The aura of success and academic dedication has faded.

It is also because of this experience that the first thing Yang Rui did when he returned to the 1980s was to solve his own financial difficulties.

And when the economic predicament was resolved, Yang Rui no longer had any scruples——

Even when he faced daunting primer design.

To be honest, for this work, the help Yang Rui can get from the database in his mind is very limited, at most he can get some theoretical guidance.

Of course, theoretical support is also valuable. The theoretical guidance not only pointed out the direction for Yang Rui and narrowed the scope of his attempts, but also gave him great confidence.

Even so, the actual operation is still difficult.

At least it is many times more difficult than for scholars 30 years later to make unknown primers.

Scholars of later generations at least have primer design software available, and this alone does not know how much time will be saved. Scholars of later generations have more templates for reference...

In 1985, Yang Rui couldn't even pursue the efficiency of the instrument.

However, Yang Rui has no fear of difficulties at all.

He has enough food and clothing, beef to eat, custom-made windbreakers to wear, wants to ride a car, wants to ride a bicycle, he also saves two houses of stamps, has his own laboratory and valuable patented technology.

Even if he puts all his time and energy into primer design, even if it takes months or even years, Yang Rui doesn't feel daunted by the difficulty.

What's more, he is not working alone.

After all, Yang Rui is in charge of the genetic engineering laboratory, and the 8 researchers in the genetic engineering laboratory are also invaluable helpers.

Scholars who do primer design in later generations are not good. Many biological experts have experience in primer design. It is true that they have more technical means and data to back them up, but it is good for scholars who do such research to have an assistant. , if you want 8 professional scientific researchers to contribute 110 hours a week to help, it is out of the question.

In the end, the God of Science heard Yang Rui's prayer, and after absorbing the blood, tears and time of eight researchers, he gave back to the genetic engineering laboratory, making up for the shortcomings in technology and equipment a little.

The only thing Yang Rui can guarantee is to keep his researchers from suffering.

Although he can't give extra wages to researchers in public laboratories, it is always easy to follow the example of current state-owned enterprises and provide more benefits.

Enterprises like China Animal Husbandry are also willing to meet Yang Rui's request.

Taking advantage of the time when the researchers were gathered around the experimental table, Yang Rui made a few phone calls, turned around, and said with a smile: "I have reported my congratulations to Director Mei, Director Tie and Director Qu, and I will invite them to come and see after the appointment." Demonstration. In addition, Director Mei promised to provide us with another cow as a gift, of course, it was slaughtered."

The researchers in the lab laughed.

"Director Mei is a good person, and he sent beef again."

"Our family hasn't finished eating the beef last time."

"You don't know how to eat. Let me tell you that what Zhongmu sends is fat beef. Don't just cook it, it's also delicious in the oven. It's the stove we use for heating in winter. Does your house have an oven?"

When it comes to eating, the atmosphere on the experimental table suddenly becomes harmonious.

Yang Rui was also full of smiles, and after several people read the experiment records in turn, he signaled to turn on the electrophoresis device again.

Primers are invisible to the naked eye, and don’t count on weaker microscopes. Furthermore, even if you see them, you can’t judge actg. Therefore, the way to verify whether the primers are successful is gel imaging. In the words of a biological laboratory, it is Run electrophoresis.

Electrophoresis can not only see whether the primers meet the requirements, but also how effective the primers are, which is the so-called specificity.

Yang Rui had already done it just now, and now he handed over the work to Gu Qiang and asked him to check it again.

Gu Qiang's experimental operation is not good, the accuracy is an order of magnitude higher than that of Yang Rui, and everyone believes in his operation, watching Gu Qiang's movements one by one, and getting nervous again.

Yang Rui was also inexplicably nervous.

False-positive data in the laboratory is nothing more than normal. Contaminated materials, or operating errors, etc., emerge in endlessly. The famous penicillin, in fact, is that the petri dish used for the test is contaminated and falls in some laboratories. There must be an experimental dog hung up on the altar to let the blood out.

Although Yang Rui used the information in his mind to design the primers this time, he did not have any specific details.

Therefore, the primers that Gu Qiang was going to test now were designed by Yang Rui independently.

This skill, even after 20 years, is better than ordinary doctor-level head dogs. However, the independent design means that Yang Rui can't compare the data and confirm it again and again, so he can't help but feel a little uneasy at this time.

If Gu Qiang did gel imaging, the specific bands of the electropherogram of the product were not obvious, or there was a dimer band, it would be a shame.

However, embarrassment is also embarrassment, and scientific research is always embarrassing.

Yang Rui watched Gu Qiang operate the electrophoresis device in a strong rhythm, but slowly relaxed.

Speaking of which, when he first went to the laboratory of Professor Tang Zhongji, what he did most every day was the electrophoresis instrument. It is not easy to say that the operation is so delicate, but it is not easy to make mistakes.

Gu Qiang's operation is naturally better, just looking at his movements gives people a clean feeling.

When the electrophoresis map of the pcr product came out, the bands were even clearer...

However, everyone's attention was only on that specific band.

"The output is so high..." Several people praised it.

The bands shown in the electrophoresis diagram of the pcr product are "barcodes" on a piece of pitch black. The "barcodes" are white, long and short, and arranged in sequence.

The clearer the barcode strip, the more content of the substance.

As far as the current experiment is concerned, the clearer the specific bands, the better, and the fewer dimers or hairpin structures, the better.

Yang Rui couldn't help but raise his head up, this was completely his design.

"Professor Ouyang, shall we do a set of appraisals?" Yang Rui's self-confidence increased greatly.

Ouyang Shi responded loudly.

Sun Zhengchu was filled with envy when he heard this, and said, "If this experiment is done, I'm afraid it can be published in top journals of animal husbandry abroad. Journal of Nutrition ( ) or British Journal of Nutrition?"

Yang Rui smiled and said nothing. Although the Journal of Nutrition is the top journal in animal husbandry, it is not the top choice for him.

"Let's see how the experiment results are first." Yang Rui didn't say much, and returned to the state of doing experiments.

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