Rebirth of the God-level Xueba

Su Xiankai became more and more proficient, and soon became an expert in bovine rhodopsin protein.

The life of the laboratory is actually like this. When the laboratory is just beginning to decide what to do, most of the participants are actually blind and can only learn by doing in the subsequent research.

This is probably not what ordinary people imagine, or in other words, it is completely different from life in industry and politics.

Repetition is probably one of the most accustomed ways of working in industry and politics. For example, in a private company, the employees he most wants are experienced employees. What kind of experience? Have done a certain project, or are familiar with a certain work, so as to facilitate repeated experience.

But in academia, this is not the case.

Except for some small laboratories or individuals who will work on certain projects for many years, the requirements for the expansion of the academic world, or the requirements for opening up new fields are much higher.

Therefore, most academic researchers are actually in a state of lifelong learning and constantly trying new projects and jobs.

This is even more so in advanced laboratories. Not to mention cutting-edge science, they are always new gadgets. Among these things, there are inevitably some familiar ones, but there are also unavoidable ones.

Learn to do it when you encounter uncommon ones, read books or do experimental training by yourself, and finally become familiar with it, and a project may be over, and then repeat it in a cycle.

Su Xiankai has never made bovine rhodopsin protein before, and there are not many in the world. If you want to talk about learning, there is no place to learn. It is not bad to teach two or three kinds of protein in school. If you teach each kind of protein, not to mention the textbook Whether it can be made up or not, there is no need to think about such things as there is no one with a lifespan of 800 years.

However, you have to do it without learning it, and just think about it yourself, which is the most common situation.

One of the effects of repeated experiments is the high learning efficiency. If it is a follower laboratory, it is the most cost-effective to train troops through various repeated experiments.

Of course, pondering and learning also have a direction.

Without Yang Rui's restriction, Su Xiankai would prefer to study in-depth research on the bovine rhodopsin protein itself, to understand its working mechanism and function.

However, Yang Rui's order was simply tiresome.

It's always pure production.

Production doubled.

Increase production by five times.

When it was ten times higher, the order was still to increase production.

Su Xiankai's research direction can only be to continuously increase production.

For biologists, increasing production has never been a mysterious topic, and the smaller the unit, the more so. For example, penicillin, which is often used as an example, was produced in tens of kilograms in China in the early 1950s, so that the price of 200,000 units of penicillin reached the level of a tael of gold. By the 1980s, China’s penicillin production How much is it? Hundreds... The unit is tons, and the pricing for patients has also dropped to one or two cents a bottle.

Without him, the production of penicillin is caused by the upward growth.

Bovine rhodopsin, in essence, is no different from penicillin.

It is even very similar to the coenzyme q1o that Yang Rui made in the first place.

Once, when Yang Rui was at the Xibao Meat Factory, the production method of coenzyme q1o,

It's the tissue extraction method, that is, get some pig heart and beef heart and mix it up, and then use various methods to extract the desired coenzyme q1o.

Later, including the West Fort Meat Factory, they all improved the scheme and adopted the plant extraction method, which greatly reduced the production cost. The subsequent semi-microbial extraction scheme and even the fully synthetic extraction scheme are also options that can be made with technological progress.

For bovine rhodopsin protein, there is only one solution, the tissue extraction method.

However, compared to commercial products such as coenzyme q1o, research materials such as bovine rhodopsin protein do not need to be too demanding on the cost.

Su Xiankai asked for an increase in the supply of fresh ox eyes, and at the same time began to gradually change the solution used for extraction, and even took re-extraction measures... When it comes to specific details, it is naturally very complicated, but in essence it is actually quite simple. The oil is similar, one is to increase the total amount of extract, and the other is to reduce the loss.

Moreover, just like oil extraction requires a refining process, bovine rhodopsin protein also requires a purification process.

It took Su Xiankai almost a month to finally enter the fast lane.

The increase in output has also increased from ten times to 50 times.

In this way, the Ion Channel Laboratory of Peking University has officially entered a node-the production of raw materials has finally exceeded the consumption.

Although Gu Qiang, Fan Zhenlong, Wang Sisheng, and two scientific research dogs were extremely busy, in the end, the five of them could not consume so much bovine rhodopsin protein.

Naturally, Yang Rui would not let it go lightly, and forced him to order: "I will give you a buffer time this week. Everyone will work overtime to use up the bovine rhodopsin protein made by Lao Su's group. Starting next week, I hope you can think of a better way to speed up the experiment process."

The faster the experiment progresses, the greater the consumption of raw materials. Of course, there are some crooked methods, but in such a small laboratory, this is not done.

Fan Zhenlong had a bitter face, saying that the baby could not do it, or he would add someone to the baby.

Yang Rui said without sympathy: "The increase in manpower can only be for the better-performing group. The progress on your side is obviously slowing down. This cannot be solved by increasing manpower. On the contrary, you have to find ways to improve efficiency."

Gu Qiang, Fan Zhenlong, and Wang Sisheng, together with two scientific research dogs, had to work overtime in a well-behaved manner before thinking about it.

However, they work overtime

In any case, the time is not as good as that of Su Xiankai.

In order to achieve 100 times the production capacity, Su Xiankai has long been freed from the 110-hour work system. He works more than 130 hours a week. Occasionally, if he only works 15 hours a day, he will be too excited to sleep.

The output of raw materials has increased, and the follow-up experimental group has no choice but to keep up.

The conformation of the transmembrane region of g protein-coupled receptors has finally been raised step by step.

To obtain a high-resolution three-dimensional structure, it is said that there are three such steps.

The first step is to have a conformation.

Conformation is a special term in organic chemistry. It is a high-end version of hydrogen dioxide. In terms of space, it indicates where the hydrogen is, where the oxygen is, and preferably the angle between them.

The second step is to obtain the crystal structure.

The third step is to obtain a high-resolution three-dimensional structure.

Any one of these three steps is an S-level achievement that can cause a sensation.

In fact, except for Yang Rui, the goals of other international laboratories are all completed in one step.

No one wants to go through three steps at once, or even go further to study the signal problem.

Because of these three steps, except the first step is a bit reluctant, each step is a possible Nobel Prize-winning achievement. Who would give up such a huge temptation to study the next step in secret?

However, the temptation to draw three conclusions is beyond doubt.

Assuming that the Nobel Prize is awarded to the three-dimensional structure of g-protein coupled receptors, those who are eligible to accept the award should include the completers of the crystal structure of the second step, the three-dimensional structure of the third part, and the subsequent signal transmission If one of the three completes the first step at the same time, he will undoubtedly become the number one award-winning scholar. If the three are completed together, it is possible to win the award alone.

Of course, since the 1970s, there have been fewer and fewer Nobel Prizes awarded alone. They even tried to award two unrelated achievements together in order to satisfy the number of three people.

The number of people who are indeed eligible to win the Nobel Prize has increased greatly in these years.

However, g protein-coupled receptors are still a strong field. If there is anyone who can win the Nobel Prize alone, it can only be such a project.

Although Yang Rui knew it was impossible, he still didn't want to give up at the beginning.

After all, foreign laboratories are not strong enough yet, and the ion channel laboratory of Peking University still has some opportunities, even if they are very small, they can still be called opportunities.

Besides, if it is trivial, the idea of ​​making a g-protein coupled receptor is very trivial, let alone the subsequent steps.

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