Rebirth of the God-level Xueba

Sleepwalking Dafa is often heard in science stories, most of which are emotionally touched by the scene, plus feelings in sleep.

The discovery of the benzene ring, the invention of PCR, and even the discovery of the DNA double helix are all successful cases of sleepwalking.

The researcher dreams back at midnight, and when he wakes up, he solves the problem that he has studied for a long time. This kind of epiphany-like mode is very popular in various scientific stories, and ordinary people also pay for it. He secretly One of the messages contained is that ordinary people can actually make outstanding scientific research results.

Therefore, young, middle-aged and old people who are interested in science will laugh at the sleepwalking stories of scientists.

Of course, ordinary people can't actually make outstanding scientific research results.

Ordinary people can make some small inventions, add an eraser to a pencil, and so on, without any problems, but the coverage of basic science has exceeded the ordinary level. Not to mention the actual discovery process, still follow the scientific process.

However, the second of sleepwalking Dafa is that it is a reasonable and legal reverse deduction-the so-called double helix is ​​very cool, so I tried it. It turns out that the double helix is ​​really good, and it is the best guide for sleepwalking Dafa.

Yang Rui hopes to make the three-dimensional structure of G protein-coupled receptors. The first step is to make a conformation of the transmembrane region of G protein-coupled receptors.

To put it bluntly, such a conformation is worth half a Nobel Prize.

If you have enough basic biologists, it is almost enough to win the Nobel Prize for the conformation of the transmembrane region of a G protein-coupled receptor. Of course, it is right to share it with others.

It is such an important scientific achievement, but when the conformation of the transmembrane region of G protein-coupled receptors is shown to the public, it is a very simple picture.

Just like a DNA double helix, which is wound by two snakes, the conformation of the transmembrane region of a G protein-coupled receptor can almost be described as an image of seven springs stirred together.

"Molecule capable of transmembrane seven times" is a protein molecule as powerful as heptadom, perhaps the most powerful molecule in the human body, but it is ultimately composed of seven tentacles, each of which is like a thick spring wire Yes, the image is really not good-looking.

However, such an image is not something you can have if you want it.

The tentacle monster composed of seven tentacles is easy to draw, but the structure of the G protein-coupled receptor has to withstand scrutiny.

What Yang Rui is going to use is sleepwalking Dafa, not really planning to sleepwalk, it is in line with the current cognition and judgment of the scientific community.

This is what Yang Rui was looking for.

He had to see what basis he had for "sleepwalking".

The three intracellular rings and the three extracellular rings were figured out in the 1950s and 1960s. The first two intracellular rings were relatively small, and the third large intracellular ring was not determined until several years later. In terms of transmembrane helical sheets of receptors, it is relatively new to recognize that helices I-IV form one sheet and helices V-VII form another sheet.

In addition, the most important thing is the image data from the electron microscope and X-ray.

Even though a protein is such a small unit, technology from the 80s is good enough to get a rough idea of ​​what it looks like.

However, the transmembrane conformation of G protein-coupled receptors focuses on the word transmembrane.

Scholars most want to figure out why G protein-coupled receptors can cross membranes, and they span seven membranes.

This is also the core problem that needs to be solved for its transmembrane conformation.

While looking at the materials, Yang Rui made sketches on the paper, but it was completely opposite to what other scholars did.

Scholars in this era, to study the transmembrane conformation of G protein-coupled receptors from scratch, first imagine the arrangement of the seven antennae in their minds, then consider the relationship between them, and then examine their relationship with the existing ones. Whether the known structural information matches, and explain the parts that do not match...

This is a very tedious and even infuriating job.

The DNA double-helix structure has only two strands, so many laboratories have played with it. G protein-coupled receptors have seven strands, and the difficulties can be imagined.

However, what Yang Rui has to do is not addition, but subtraction.

After 30 years, the transmembrane conformation of G protein-coupled receptors is known, and the three-dimensional structure is known, not to mention the conformation of the first step.

But Yang Rui couldn't directly take out the conformation of the later generations, because of certain restrictions, the current academic circle doesn't know yet.

At the same time, Yang Rui also needs to confirm that his laboratory's research progress has reached a corresponding level. It is best to infer the conformational data from the research progress of his laboratory--it is said that he wants to draw a picture, but in the final analysis, all Images are expressed digitally.

And numbers, numbers naturally have a basis.

Yang Rui dug out all the materials, and only managed to make up 70% to 80% of the content. As for the rest... naturally, he had to force other people to do it.

"When I was sleeping last night, I thought about it carefully. The transfer of the seven helical chains must have a certain effect. I tend to think that they are used to recognize substrates, and they are specific substrates. Molecules..." Yang Rui said while writing two lines on the blackboard in the laboratory:

1. Determine the contacts formed by the 7 helices and the substrate molecule.

2. Describe the local conformation according to the substrate molecule.

The simple twenty or thirty words are like a container falling on the jeep, making everyone breathless.

"Did you think about these things when you were sleeping?" Gu Qiang looked at the two questions and wanted to complain.

Yang Rui nodded steadily. Sleepwalking Dafa is good. The good thing is that it is easy to explain the ideas and avoid a series of logical problems.

Gu Qiang was very helpless, curled his lips and said, "I wonder how you can sleep."

Yang Rui coughed twice, and said: "If you give the answer, I will definitely be able to sleep."

"Is there no ready-made solution?" Professor Man Hai asked.

"No."

"So, everything depends on testing?"

Yang Rui shrugged.

To test the contact between seven helices and substrate molecules, it is natural to use substrate molecules and G protein-coupled receptors to test. There are hundreds of substrate molecules, and the test is naturally tens of thousands.

Professor Man Hai felt his eyes go dark, and murmured, "No wonder you asked them to make so many bovine rhodopsin proteins."

"Now is still our advantage period. Everyone, please work hard. When the projects are all over, everyone will be able to go abroad." Although Academician Zhu came forward to do the work, the trip abroad was rejected, which was still very demoralizing. Yang Rui could only take the opportunity to draw cakes.

Man Hai also took the opportunity to show his loyalty, saying: "As long as it can be done, it doesn't matter whether you go abroad or not."

"Creating results is the focus now." Yang Rui quickly pulled the topic over and said: "I have some preliminary ideas about the conformation of G protein-coupled receptors, and I hope everyone can do their best. work at hand, contribute to the completion of the project..."

Yang Rui didn't take out the whole composition of his sleepwalking flow, but he still made a few simple partial pictures and handed them to several researchers.

A group of people began to study in silence, some who couldn't understand stared blankly, and some who understood, blankly began to search for old materials for comparison.

Yang Rui didn't care about it, instead he felt a little drunk after drinking.