Rebirth of the God-level Xueba

"Director Yang, what's wrong?" Gu Qiang scratched his head.

"It's because it's so suitable." Yang Rui muttered to himself, "Did you generate the complex in the presence of detergent?"

"Yes, the args complex you named, I have tested, and it can effectively couple in the phospholipid bilayer."

Yang Rui looked up at Gu Qiang, and asked again: "Is there no decrease in coupling efficiency? I mean, in the presence of detergent."

In the first phase of research, detergents were also used to treat g protein-coupled receptors, but it was different from the second phase. In order to ensure the "purity" of g protein coupled receptors, the second phase always required The presence of detergents is a relatively high requirement. The most important thing is that once there are detergents, the coupling efficiency of g protein-coupled receptors will be extremely reduced, and it will drop to an appalling level. the point.

There are two ways to solve this problem. One is to complete a series of experiments before the coupling efficiency drops to unacceptable, and the other is to solve the problem of coupling efficiency decline, or at least extend the time of high efficiency.

Yang Rui asked for Gu Qiang's experiment records and searched them.

I saw Gu Qiang's experimental record, and this part of the record is only a short page.

Gu Qiang's answer was even simpler: "There was a drop in the initial stage. After I changed the solution, the problem of the drop in coupling efficiency was solved."

Yang Rui's voice was about to tremble, and he pointed to Gu Qiang's experiment record, and said, "You changed the dodecyl maltoside solution."

"Yes. However, the complex thus formed was later found to be unstable."

"Yeah, it must be unstable."

Gu Qiang nodded, and said: "Then I remembered the experiment I did before, and when I encountered similar situations, I used enzymes to solve them, so I added adenosine triphosphate phosphatase, just to put the complex on, and give gdp to it. Hydrolyzed down to avoid interaction with gs."

"Yes, it happened to be hydrolyzed." Yang Rui was speechless.

Gu Qiang said with a guilty conscience: "Is there something wrong? I did everything according to your plan, and there are no side effects."

"The problem is that nothing went wrong." Yang Rui stared at Gu Qiang for a long time, and said, "How did you make the plan so smoothly?"

"Isn't it good to be Shun Shun Dang?" Gu Qiang has a somewhat rebellious character, but he has been tamed by Yang Rui long ago, and now he is a little uneasy.

Yang Rui recalled Gu Qiang's counter-intuitive development of "one pot stirring" technology, and his precise and impractical experimental skills, and suddenly asked, "Have you checked Moments?"

Gu Qiang was stunned for a moment, and said: "I have no friends to play tricks on me."

"Okay." Yang Rui sighed, and said, "Congratulations, another top-level paper has been produced by you."

"Make it?" Gu Qiang was stunned.

"Yes, it's done." Yang Rui confirmed.

"Is it really done?"

"It really worked out."

"Impossible, it's only been a few days." Gu Qiang jumped excitedly, his cheeks flushed red.

Yang Rui nodded in agreement and said, "I also think it's impossible,

It's only been a few days. "

"Even if the second stage is completed?"

"Is this the crystal structure of the complex?"

"The crystal structure is made?"

Affected by Gu Qiang's cheers, a group of researchers surrounded him.

Like a tour guide, Yang Rui told a passionate story flatly, saying: "Although some mathematical calculations and verifications are still needed, the crystal structure is almost done."

A group of people looked at each other in blank dismay, and finally came down to one question: "How is it possible!"

Yang Rui sighed empathetically, not knowing how to explain it.

To say that this story that is destined to become a wonderful anecdote is also inseparable from Yang Rui. It is he who gave the customs clearance cheats and gave out the whole set of plans.

Yang Rui did this to increase the speed and complete the second phase of research as quickly as possible. Given a complete set of plans, the researchers will be able to make targeted modifications and tests.

However, given the full set of plans, it took a few days for someone to complete the plan. This is the most unbelievable thing.

Let alone a Nobel Prize-level scientific research experiment, even a junior high school experiment, the teacher still gave a complete set of experimental plans. Could it be that all students can produce results?

Find a middle school chemistry teacher to answer this question. He can tell a hundred kinds of strange experimental results, none of which are real experimental results.

For a slightly more difficult university experiment, how many students can produce results? There is one out of three, even if the students are studying hard.

In weaker universities, it is very common for a class to fail to produce a single result in an organic chemistry experiment class.

Not to mention the difficulty of experimenting with the crystal structure of g protein-coupled receptors. Among the key points, the first is to use the correct detergent and control a relatively appropriate concentration - the requirements are relatively broad and easy to achieve , but just for this point, it is not something that can be done by pulling a random experimental dog.

Next, complex crystals of g protein-coupled receptors should be generated at an appropriate concentration.

There are even fewer researchers who can make this step. If this is used as a graduation requirement for university experimental courses, 90% of undergraduates will cry.

Then, in the presence of detergent, the coupling of the composite crystals is ensured, and

It is a requirement.

Historically, KBKA found that dodecyl maltoside solution could solve the coupling problem. There is no record of how long it took him to do this, but with full malicious speculation, it is trivial to try for three or four years. Of course, it wasn't KBka who tried it himself. He has many doctoral students and assistant researchers, but this actually only shows how many excellent researchers' scientific lives were wasted.

After solving the conjugation, the remaining steps, including enzymatic hydrolysis, etc., are not particularly time-consuming, but as far as the normal historical process is concerned, it always takes several months. When Yang Rui operates the golden finger, he also reserves the time according to two months.

And yet, all these, all these golden fingers, the flashes of genius, the painstaking efforts of hard-working scholars, are as indistinct as smoke and dust under the luck of shit.

Gu Qiang did it step by step, making more correct steps than trial and error steps, doing it, coming out...

From Yang Rui's point of view, it's as if you installed an extremely difficult stand-alone game, found a strategy for clearing the level from the Internet, and then... cleared the level with three lives!

"Let me take a look again." Yang Rui called the names, and said: "Su Xiankai, Fan Zhenlong, Tian Bing, that...Professor Man Hai, Professor Tao Xuelin, you also join in, and follow Gu Qiang's experimental procedures to do a repeated experiment. Old Xu, you and I check the experimental records again, by the way, the experimental table is not allowed to move, and the garbage is not allowed to be dumped, and the packaging of all used consumables must be recorded."

The researchers whose names were called all gathered together.

Gu Qiang still didn't realize his strong luck today, so he laughed and said, "I don't need so many people, my experiment is easy to do."

"Well, Gu Qiang, you open another set and do a set of repeated experiments." Yang Rui didn't dare to take it lightly. This is another Nobel Prize-winning achievement, and it was done in a few days. Who would believe it?

Gu Qiang changed the experimental table indifferently, took all kinds of consumables again, started to work, and said in his mouth: "If you say anything else, I'm afraid, and I'm the least afraid of doing experiments."

While talking, Gu Qiang also hummed a song.

Only Yang Rui stared at the various experimental tables in the laboratory as if facing an enemy, his mind turned.

The progress chart on the white wall is brand new, only at the bottom, two more participating laboratories have been added, namely the University of Melbourne in Australia and the Winter Institute in the United States. The progress bars are 27 points and 16 points respectively.

Yang Rui glanced at it, and stopped looking at it. If the triple repeated tests can prove the accuracy of the results, the progress bar of Peking University's ion channel laboratory will be approved as 100 points.

...