Rebirth of the God-level Xueba

"Where did you fail?" Yang Rui wasn't very nervous, but just froze for a moment, then continued to brush his teeth.

Gu Qiang looked ashamed, and said: "The dissociation or purification is not clean, and the image is gray."

As he spoke, Gu Qiang rubbed his head vigorously, and said, "I've done purification countless times, but I didn't expect something to go wrong this time."

"Are the steps before purification correct?" Yang Rui asked with foam in his mouth.

"It should be fine, I checked it before." Periodic checks are a necessary means, otherwise, if the pre-experiment crashes only later, the experimenter himself will have to crash.

Yang Rui nodded, and said: "Well, first, you check yourself to see where the mistakes are."

Gu Qiang let out an "en" with a muffled head.

"Secondly, I will form another team to help you do the inspection, mainly to check the experimental records, and compare them with your previous experiments to see if there are any differences." Yang Rui said very generously, which is the scale of his laboratory If it is too big, otherwise, the existing three repeating groups will be able to support ordinary scientific research laboratories.

Gu Qiang still kept his head in suspense, but didn't say anything this time.

"Third." Yang Rui continued: "The speed on your side should be faster. After the inspection, no matter what the conclusion is, do another set of repetitions. Didn't I ask you to protect your experimental table before? Consumables There are also records, you can compare and see if the change in the consumables caused the difference in the experimental results..."

What Yang Rui said was extremely systematic and simple.

In the final analysis, it is just a sentence, compared with the old foundation left behind.

This is the similarity and difference brought about by whether there is a systematic scientific research experience. Gu Qiang is a semi-wild researcher who has joined the laboratory after graduating from undergraduate. Before, he thought Yang Rui's requirements were cumbersome, but now he realized the importance.

If Gu Qiang was still a little confused about the first and second points, Yang Rui's third point boosted Gu Qiang's mood more or less.

However, Gu Qiang was still a little uneasy and said: "There are only so many things in the laboratory, and it is usually impossible to make mistakes."

"There are too many things that can go wrong. Maybe a bottle of medicine has been released for too long and decomposed in the sun." Yang Rui casually mentioned an example.

In the laboratory, all brown bottles and black bottles are designed to resist sunlight. There are more reagents that can be decomposed by ultraviolet rays than people imagine, especially the solvents that are prepared in the laboratory.

The same principle is also used in bags of braised chicken legs. If you pay attention, the bags of braised chicken legs are not transparent. In fact, for the purpose of sales, it is the most effective publicity to allow consumers to directly see the food inside through the packaging bag. However, due to the decomposition of sunlight, the beautiful marinated color of the marinated chicken legs will fade after being exposed for a few days. It became pale and pale, making people lose their appetite.

There are too many error-prone links in the laboratory, especially for a long series of time, it is even more difficult to do it all at once. Because of this, Yang Rui felt that Gu Qiang's luck was unbelievable.

In Yang Rui's view, it was normal that he failed to repeat the success for the first time.

Gu Qiang frowned, waited for Yang Rui to rinse his mouth, wash his face, and came out of the bathroom, followed closely, and said: "The problem is, when I do repeated experiments, I pay attention to it, I'm worried..."

"There's nothing to worry about." Yang Rui stopped,

Turning around to look at Gu Qiang, he asked: "The crystal structure has been released, and this is the undoubted evidence. Repeat the experiment, but I want to be more cautious, otherwise, if I switch to another laboratory, in order to save time, maybe all of them are now The article is out. So, the experiment you did can definitely be repeated, we just need to find out what the problem is."

The crystal structure of a g protein-coupled receptor had never been seen before.

This is like an image of a rare creature, and it was imaged at one time with an instrument, which is evidence that iron can no longer be iron.

Gu Qiang lowered his head silently, raised his head again, and said in a low voice, "Aren't you afraid that my experiment will be fake?"

Yang Rui gave him a strange look, and said, "How could it be a fraudulent experiment?"

"You believe me?" Gu Qiang was suddenly excited.

"Of course I believe you." Yang Rui frowned: "What are you worried about?"

Gu Qiang only felt a pang in his chest, but he couldn't speak.

"Did anyone say anything?" Yang Rui asked.

Gu Qiang nodded slightly, and said: "I heard some gossip, it's nothing."

As he said that, he rushed into the bathroom, turned on the tap, washed his face well, blew his nose while washing, and after a while, he wiped his face with a paper and said, "It's okay, I'm just afraid of messing it up. "

"If you have done it, you have done it. Be more confident." Yang Rui patted Gu Qiang on the shoulder and said, "Okay, don't think too much about it. Two more rounds of inspection, a repeated experiment, how difficult it can be."

Gu Qiang clenched his teeth, nodded vigorously, and said, "I will definitely make it."

"Well, let's go."

Yang Rui was still in the state of just waking up, and didn't think too much.

He actually forgot that although he knew the crystal structure of the g-protein coupled receptor made by Gu Qiang, others did not. Even Gu Qiang, in fact, is not sure whether what he made is the real crystal structure of g-protein coupled receptor. The second stage has just begun, and everyone's knowledge reserves are not complete.

Even because the plan given by Yang Rui is so complete in one step, everyone's guesses about the crystal structure of the g-protein coupled receptor are not very complete.

In theory, Gu Qiang can make the crystal structure of any compound and treat it as a g-protein coupled receptor

The crystal structure of the body, the so-called deer is also a horse.

Of course, this kind of fraud is a very low-level fraud, but several of the scientific research frauds exposed in history are high-level. For example, a laboratory in the United States made an AIDS vaccine, injected it into rabbits, and produced antibodies. The pharmaceutical industry was shocked at the time, but the results were never repeated, so they had to admit that it was fake. This is a typical mentally handicapped falsification. Of course, it cannot be repeated. If it can be repeated, wouldn't it mean that the vaccine was successful, and it would not be called falsification.

In the ion channel laboratory, there are researchers who are familiar with Gu Qiang, and there are also assistants who are not familiar with Gu Qiang. In the case of incomplete information, it is inevitable to question Gu Qiang.

In fact, if you look at the progress chart on the white wall, you will feel that it is correct to question - the progress of the top foreign laboratories is not even 1, and Gu Qiang actually implemented Yang Rui's plan in one go. Extremely unreasonable.

After the initial admiration and shock, everyone's doubts followed.

When Gu Qiang was busy for a day and night, but declared repeated failures, doubts inevitably showed some sharp corners.

Even Gu Qiang cannot avoid doubting himself: Am I making a mistake? Am I reading this wrong? Did I get a plausible result by accident?

Back in the laboratory, Gu Qiang held his breath and started a new round of repeated experiments on his own. However, his appearance was far from his inner peace.

ah!

Horse's!

This time, before night, Gu Qiang failed again.

Hearing the sound, Yang Rui came to Gu Qiang's experiment table and asked, "Misoperation?"

"En." Gu Qiang was extremely annoyed.

"Well, let's analyze your last round of experiments first." Yang Rui called the name and said: "Professor Man Hai, Professor Tao Xuelin, you are more experienced, um, add Wu Qi, come here, let's help Gu Qiang analyze .”

Man Hai was obviously a little unhappy, and asked, "How do you want to analyze it?"

"Analyze everything from beginning to end to see the difference between successful and failed experiments." Yang Rui replied.

Man Hai was surprised: "It will take a lot of time."

"Maybe."

"Should we...don't bet all the treasures here?" Man Hai couldn't help hesitating, he didn't know Gu Qiang well, so he didn't want to waste all his precious time on it. If it turns out that there is a problem with Gu Qiang's experiment, the time it takes for a comprehensive analysis may lead the ion channel laboratory to the abyss of failure.

Yang Rui sneered at this, and said: "If you can't repeat it twice, you have to find a new way? I will give you two years, can you make a result? Is the plank road so easy to walk?"

What he said was extremely blunt, and it could even be said that it was in a critical tone. As a professor at Peking University, Man Hai blushed immediately after being trained by Yang Rui.

Gu Qiang, who was beside him, also blushed.

To him, Yang Rui's affirmation at this time can be called nectar.

"Well, comprehensive analysis, do you want to find out all the differences?" Wu Qi raised his hand cautiously, distracting the somewhat dignified atmosphere by the way.

Yang Rui turned his head and said, "That's right, all differences must be marked."

Repeated experiments are not easy experiments, and students who can't do experiments according to books should be more aware of this.

"I've seen one before, I don't know if it counts." Wu Qi looked at Yang Rui, then at Gu Qiang.

Yang Rui said kindly, "Say it."

Wu Qi swallowed a mouthful of saliva, and said: "I read the experimental records of Researcher Gu, the isoamyl alcohol he made the buffer solution for the first time was taken from the No. 3 cabinet, and the isoamyl alcohol used for the second buffer solution was taken from Take it from cabinet 4."

"What's the difference? It's a product of the same company." Gu Qiang originally had some expectations, but now he feels bored.

His own experimental table is relatively close to cabinet No. 3. After changing the table, it is even closer to cabinet No. 4. He feels that there is no need to take reagents from a specific position.

Wu Qi was a little nervous, and said: "Although it is from the same company, I don't know if it counts. The isoamyl alcohol in cabinet 4 was delivered last month, and it is specially put here for our practice. of."

Gu Qiang's face suddenly changed: "Why not mark it?"

Wu Qi whispered: "It's okay when we use it, but I don't know if it affects your experiment."

"I'll do it again." Gu Qiang didn't argue with Wu Qi, unfolded the experiment table, and started operating.

Everyone's knowledge and interest dispersed, but the topic still focused on Gu Qiang and his experiment.

...

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