Rebirth of the God-level Xueba

Enucleation of cells is difficult.

It was difficult in the 1950s, and it was also difficult in the 1980s. After the 21st century, it is still difficult.

It can be said that anyone, no matter with a bachelor’s degree, a master’s degree or a doctor’s degree, as long as he can master the method of cell enucleation, he can easily find a job with a monthly salary of 3,000 yuan in any full-time university. Work!

Like Comrade Wang Liangcai, if he goes abroad now, he can easily find a teaching position with an annual salary of 40,000 yuan.

If we use an analogy to describe it, the work of denucleating and reperfusing cells is like performing heart surgery in a hospital. Is there any operation that is more difficult than heart surgery? Of course there is.

However, in terms of popularity and popularity, heart surgery is more eye-catching.

Wang Liangcai is the chief surgeon of the cardiology department in experimental biology.

His success rate is not high, but there are not many people in the whole country who can manipulate the position of the cells little by little under the microscope, and then carefully suck out the nucleus.

Wang Liangcai worked in the laboratory for a few days, and everyone expressed their admiration.

The laboratory, like the factory, is the place where the ability can be best reflected.

Because everyone makes real objects, and the workplace is in the same place, so there is no need for you to show off whether the things you make are good or not, anyone who opens their eyes can see them.

In fact, in order to show their vision, those who can see have to help with publicity.

Therefore, a good strongman in the laboratory is like a good eighth-level and seventh-level worker in a factory, it is easy to be discovered, and then it is easy to be praised.

Coupled with Wang Liangcai's older age and humble personality, it didn't take long for even scientific researchers to get used to asking him for advice.

"Professor Wang, can you take a look at this for me?"

"Professor Wang, there is always a white spot in the image I scanned electronically, can you help me look at it?"

"Professor Wang, I have an idea recently, it's like this..."

The voice of "Professor Wang" came to my ears, but Gu Qiang was extremely uncomfortable listening.

Once upon a time, these questions were all asked by him, Gu Qiang... At least, some of the questions were asked by him, Gu Qiang.

Although I thought it was quite troublesome at the time, but no matter how troublesome it is, it is my own dog. What's the matter with adding other people's thighs now?

"Professor, I want to apply for more time." Gu Qiang couldn't hold back, and went around the large work area to discuss policies with Yang Rui.

Yang Rui looked down at the expression of the paper forever, only raised his eyelids, and asked: "Your phone is running out of time? What kind?"

"Inverted phase-contrast microscope, besides, pulse instrument, I also want a little more time." Gu Qiang replied quickly.

"Oh. How much do you want?" Yang Rui tapped the table with his hand and put down the paper.

It's normal to run out of time. It is impossible for any laboratory to supply an unlimited amount of instruments, and even public laboratories rarely purchase high-priced instruments repeatedly.

In this case, sharing the machine is the best solution.

Of course, if Yang Rui was working on the project by himself, he would have to use the machine as soon as possible, but it is impossible for Gu Qiang to have such good conditions for the experiment.

He has to apply for a slot like other researchers, and then conduct experiments within the specified time. If it cannot be completed by the due date, nor can it be occupied beyond the time limit, it must be discussed with subsequent researchers.

Or, for now, apply for more slots.

Gu Qiang glanced at Wang Liangcai and said, "Four hours of microscope, two hours of pulse instrument."

There is only an inverted phase-contrast microscope in the laboratory, and Yang Rui didn't even need to look at it, just said: "Four hours are gone. If you work during the day, I will give you two hours at most."

"That's not enough." Of course Gu Qiang knew that he didn't have such a long time. The inverted phase-contrast microscope is currently a high-end product. If you use it to write papers, it will be like playing with an SCI in half a month, although it is a waste of money to do so.

However, in a genetic engineering laboratory,

The usage rate of the inverted phase-contrast microscope is already high. In addition, Wang Liangcai and others are actively working, and it is basically impossible to spare 4 hours.

Yang Rui shrugged and said, "You can follow Wang Liangcai's example and stay later at night, or come earlier."

No matter how expensive the microscope is, it cannot be compared with supercomputers and the like. In addition, the genetic engineering laboratory has not expanded to the level of the department, and there is no machine time in the early morning. Wang Liangcai rushed over early, which was equivalent to grabbing an extra opportunity.

Gu Qiang wanted to compete with Wang Liangcai, shook his head without hesitation, and said, "I want to do a set of coherent experiments, I want to connect the time of day, if you give me a few hours, I will take a week. "

"Do you have an idea for an experiment?" Yang Rui looked at Gu Qiang in surprise.

Gu Qiang toughened his scalp and said: "With it, it's just a matter of verification."

"Is it enough to add 4 hours?" Yang Rui still has confidence in Gu Qiang's experimental ability.

Gu Qiang continued to bite the bullet and say yes.

Yang Rui hesitated for a moment, and said: "If this is the case, you have taken over other people's opportunities. Wait for me to ask."

Gu Qiang couldn't help feeling helpless. He still needs to discuss with other people, most importantly, he also needs to discuss with Wang Liangcai, such a result is not what he wants.

Yang Rui did not call Wang Liangcai over to inquire, but went directly to Wang Liangcai's test bench and watched his operation.

Wang Liangcai is doing the operation of displaying the core.

This is the first step in enucleation. You have to make the nucleus of the cell visible before you can aspirate it.

If the enucleation experiment is likened to a happy amusement park, nucleation is the step of buying tickets and queuing up. Do the fun part.

Sadly, the denuclearization experiment is not a happy playground, it is boring to death, and the part of imagining the nucleation is so boring.

However, what Wang Liangcai did was meticulous, and what Yang Rui saw was also meticulous.

With him by his side, no one else came over.

The two of them watched each other for nearly an hour.

Yang Rui watched the part showing the nucleus from front to back, and couldn't help feeling a little emotional.

In another 20 or 30 years, there will be no fundamental changes in the denuclearization process. By that time, how many people will be able to do it as well as Wang Liangcai?

The biology department of each school basically recruits one or two classes of genetics students every year. After a year of accumulation, after 30 years, every school will have at least one cell enucleation experiment. researcher?

That is naturally impossible.

Just like most hospitals, there are no good cardiologists.

They may have one or two good doctors who can do it, and thus build a decent department or two, just like most schools have one or two good majors.

But that's about it.

As for Wang Liangcai's operation, it was far beyond what he could handle.

Relying on the level of experimental biology, how good are the people who have entered the selection of academicians? If you tie a sheep with a white face and tickle it, he can make the sheep laugh for a lifetime.

"It's all standard operation." This is why Yang Rui sighed.

It seems that everyone knows the standard operation, but if you really ask, how many people can really do it all.

Just like athletes on a basketball court, can professional players perform standard operations?

If so, are the species that shoot less than 50% from the free throw line grown on trees?

Compared with players like Gu Qiang who rely on talent, practicing standard operations step by step not only requires more resources, but also means more dedication.

Sometimes, you can make good experimental results without using standard methods, and you can change all your operating habits, so you have to be cruel to yourself.

Wang Liangcai didn't notice the appreciation attached to Yang Rui's semantics, and smiled and said: "It's like this if you do too much. When we were in school, we had nothing to do, so we went to the laboratory to peel eggs. If the test bench is not enough, we will prepare for the experiment. Come and go. are familiar."

"I see your experiment records, the success rate is 30%?" Just like there is a notebook next to the hospital bed, there is also a notebook next to the test bench, which is used to record simple data.

Of course, those who can record such details must be high-end laboratories such as genetic engineering laboratories. Ordinary laboratories are like going to hospitals in cities and counties. It is good to have a dirty notebook. up.

Wang Liangcai was neither proud nor discouraged by the 30% success rate, and said calmly: "Yes, I want to achieve a success rate of about 50%, and then proceed to the next step."

Nucleation, denucleation and injection are said to be three major steps, and they must be completed in one go.

According to the experience of the predecessors, if these three steps have a success rate of a few percent, it is considered good.

However, the success rate is calculated by product. If Wang Liangcai's success rate of each step is 30%, then after three steps, only 2.7% are left, which can reach the passing line.

Considering that there will be more difficulties in the future, and those who can strive for a high success rate in the front, we still need to strive for more.

Yang Rui nodded and said, "You did a good job. I heard that you often come to the laboratory in the early morning?"

"Yes..." Wang Liangcai did not answer firmly.

Coming early is not necessarily a good thing. The discipline of the laboratory is all kinds of strange things. As a seconded person, he is always a little uncertain.

Besides, is it guaranteed to be appreciated if you go to the gym at four o'clock in the morning to strike iron? What if it affects the lingering relationship between the coach and the manager?

"I want to adjust the machine, if you are willing to come to the laboratory earlier, how about I leave the morning time for you?" Yang Rui's answer was somewhat unexpected to Wang Liangcai.

"can you give me?"

"Well, just according to your previous machine time, or do you want more?"

"Can I ask for more?"

"I'll give you up to one o'clock at noon, is that okay?"

"Okay." Wang Liangcai was overjoyed.

The morning time is undoubtedly the best time to experiment. Quiet aside, all the instruments are in the best condition, because at the end of the day's experiment, all the instruments that can be reset to zero must be reset to zero, and there is less time for adjustment the next day, which saves time and saves time. Comfortable.

A few days ago, Wang Liangcai came to the laboratory in the early morning, and at 8 o'clock in the morning, he had to free up microscopes such as inverted phase-contrast microscopes that were allocated during the day. say good things.

Now that the time is connected, it will be much more convenient for him.

Gu Qiang was completely helpless listening to it, he knew that Yang Rui readjusted the time to make room for him, but...why didn't you give me time in the morning?

"It seems that the time has come to prove yourself with strength." Gu Qiang clenched his fist silently.