Rebirth of the God-level Xueba

"What happened?" All the people in the laboratory naturally gathered together. Le Wen ▸ Novels |

Huang Mao turned an electronic screen on the experimental bench, and said: "I saw a significant flow of potassium electrons before, and I confirmed that I detected it again just now. Look at the records. The average single conductance exceeds 250 psi per meter. Ten times larger than the single-channel conductance of ordinary potassium ion channels."

Pisi per meter is the unit of conductivity, and it is also one of the inspection marks of a single channel. Ten times the conductivity is definitely abnormal, and everyone immediately gathered around to look at the recording paper.

Yang Rui stared at it for a while before realizing what had happened.

This appears to be a trait encoded by family genes.

Yang Rui searched quickly in his mind, and found 4 members of the family, and then he was a little dumbfounded.

Huang Mao properly found the first member channel of the family.

This is two different things from the shaker channel that Yang Rui imagined.

The shaker channel is the first potassium ion channel identified in history, and also the first potassium ion channel identified by gene cloning. There are quite a few papers describing it, and it's relatively simple to do.

Yang Rui originally thought about making a shaker channel first, and it was easy to add some things to form a standard paper.

Even, copy a paragraph of the Shaker channel paper that will actually be published in 1987, and maybe it will be published in "Nature".

However, scientific research is sometimes so unprepared.

It is not what you want to achieve, you can achieve whatever result.

Historically, most scientific research achievements, especially breakthrough scientific research achievements, were surprises, and in many cases, they were even the result of making mistakes.

It is true that the shaker gene was the first potassium ion channel to be discovered, but its discoverer did not know what he would discover back then.

Just like Huang Mao now, although he is mutagenizing the fruit fly gene, he doesn't know which gene he will mutagenize, or in other words, after the mutagenesis, he is not sure whether he can find it.

After two or three months of experiments, until now, Huang Mao has not observed the uncontrolled shaking of the fruit fly's calf (shaker) like the scientists did back then. On the contrary, Huang Mao has found a phenomenon that is not easy to observe : The conductance increases!

certainly,

The so-called difficult to observe is also relative.

The shaker gene is also called the leg shaker gene. If there is a problem with this gene, the voltage of the potassium ion channel of the fruit fly will also have a problem. In this way, when the fruit fly is stunned by ether, the calf will keep shaking.

However, there are so many people in the Huarui laboratory, who have done hundreds of thousands of mutagenesis groups, or did not mutate the leg-shaking gene, or mutated it, and did not stun the fruit flies at that time, so no such phenomenon was observed. Phenomenon - this is perfectly normal. If the phenomenon of gene mutation is so easy to observe, the biological world will not be playing with fruit flies for decades, and it will still be playing with fruit flies in the 21st century...

In terms of the unluckyness of the group, the fruit flies have eight more lives of misfortune than the mice.

In comparison, the shaker gene is easy to observe externally, while the increase in conductance observed by Huang Mao is the easiest phenomenon to observe on instruments.

Because what we are doing is potassium ion channels, it is also necessary to observe the conductivity of potassium ions, and the channel is even more special. Its gene shows that the conductivity increases by 10 times.

In this way, even if the accuracy of the instrument is not high, a change of an order of magnitude can still be easily noticed.

"Show me the experiment record." Yang Rui was still a little excited. To determine potassium ion channels by means of mutated genes, the key point is to confirm the appearance of mutated genes.

If it is described in words, this is a very simple process: the mutation of gene a is induced, and it is found that the organism has changed b, so it is naturally determined that the function of gene a is to keep b unchanged.

For example, if the shaker gene is mutated, the fruit fly's legs shake, so it is determined that the function of the shaker gene is to keep the fruit fly's legs from shaking.

Doing this step is actually a very good paper, but it may not be able to be published in "Nature".

If you want to be sure that you will be admitted to "Nature", you have to repeat the induction process continuously, and then determine why your legs are shaking and why your legs are not shaking. In the end, it turns out that it is a voltage problem, plus a smooth article, this is basically a good paper for "Nature" up. Of course, if you want to be published in "Science", regardless of the content, the writing must be somewhat literary...

As a literary youth in the field of natural science, the hairline that "Science" pushes back can circle the earth seven and a half weeks!

Huang Mao was also excited, and quickly found a few boxes from the top cabinet, then dug out the experiment records and handed them to Yang Rui.

His experimental records are very detailed, which is also Yang Rui's request, or experience.

After 20 years, foreign laboratories have adopted video experiments to record, and the purpose is to record the experiments in detail.

People who have just finished the experiment will remember the experiment process very clearly, but after a few weeks, months, or years, the memory fades very sharply.

In the end, the research institute with a good memory may remember the main steps or minor steps of the experiment, but which company's equipment is used in the experiment? Who is the chemical reagent used in the experiment? 100 people out of 100 can't remember.

One or two of these details doesn't matter, the whole experiment will be more. If it is a long-term experiment that lasts for several years, when it is time to re-verify or re-comparison, is it possible to do the experiment again?

Even if it is done again, the chemical reagents provided by different companies are also different. If there is a deviation in the result, whoever it is.

In the experiment of potassium channels, Yang Rui first had to make sure that the experimental conditions did not change significantly when the conductivity was abnormal.

Fortunately, the conductance of the channel changes very drastically, and the 10-fold change is basically difficult to be affected by the outside world, which makes the audit work relatively easy.

When Yang Rui reviewed from beginning to end, Tu Xian and others had already started to congratulate Huang Mao.

On the contrary, Huang Mao himself was a little worried about gains and losses. While looking at Yang Rui's unchanged expression with trepidation, he smiled and said, "Look at your red eyes, the second author is also jealous?"

"Who is jealous of you? Our old Tu has also achieved results recently, hasn't he?" Wang Xiaoyun has the advantage of being a woman, so she can speak more casually.

Huang Mao deliberately laughed, and changed into a smug expression, saying: "The first paper on the Potassium channel will take a big advantage, but the second paper may not necessarily be."

"As far as your current research progress is concerned, it's not certain whether you can do the first paper, is it?" Wang Xiaoyun poked her husband.

"I just need to do the experiment. Yang Rui will be in charge of the thesis." Tu Xian ignored his wife's aggressiveness.

Wang Xiaoyun punched him helplessly and said, "I'm not motivated."

"Doing experiments every day is not self-motivated." Wei Zhenxue groaned strangely, and said, "You two parents-in-law are working like this, and other people's children should go to elementary school."

Wang Xiaoyun blushed suddenly and dared not speak. Both of them are busy in the laboratory, so naturally they don't have time to have babies, which is a little different from ordinary marriages in the 80s.

Instead, Tu Xian smiled and said, "Old Wei, can a single person have children?"

Wei Zhenxue's momentum was stagnant, and after thinking for a long time, he came up with a wonderful counterattack method, and said, "Do you believe that I made a test-tube baby myself?"

With the level of Huarui Laboratory, it really has the hardware conditions to do test-tube babies, and Wei Zhenxue also has the corresponding operation foundation.

However, this kind of bastard's way of speaking is only unique to Wei Zhenxue.

A normal person would at least find a **** beauty with big breasts or something.

Tu Xian was taken aback by Wei Zhenxue's unexpected answer. At this moment, Wang Xiaoyun asked coldly, "Do you use the test tube you used to hold mung bean soup?"

"Pfft..." The experimental dog Sun Ruyue, who had been afraid to speak until now, couldn't help it anymore, and snickered.

Tu Xian and Huang Mao also kept coughing.

Wei Zhenxue looked as usual, and said: "I told you, the test tube contains mung bean porridge, and the separated mung bean soup."

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