The Godfather of Surgery

"I remember that Professor Guan once had a paper that involved the study of expressed proteins in tumor cells. It mentioned the modification of this expressed protein. I have read this paper published in the journal "Science" and I would like to ask. , what specific methods were used for modification and marking at that time? "

Everyone discusses with great enthusiasm. After all, they are all top experts in this industry. This kind of defense meeting itself is a process of questioning and clearing up doubts. After fierce debate, the gold content of your topic is finally determined.

That previous paper?

Guan Ruyan only knew roughly. After all, he didn't design the experiment, nor did he complete it himself. He didn't write the paper. Some of the deeper things in it would definitely not be clear.

Lu Xiaolu hasn't come yet. How long has it been? Lu Xiaolu has never been in this situation before. He always followed Guan Ruyan every academic meeting. Now he actually ran away from such an important meeting, but you can't blame him. , other things can be tolerated, but no one can tolerate diarrhea.

Guan Ruyan wanted to make a call, but because President Rao and Academician Xiang were sitting next to him, he couldn't make a call. At this time, Guan Ruyan was on pins and needles. It would definitely not work if he didn't answer the question. If he answered the question, it would be easy to reveal his secret. Everyone here is really real. It is impossible for the top experts to fool him. At this time, Professor Guan Da is simply frying in a frying pan.

"We are using a method at the intersection of click chemistry and bioorthogonal chemistry. Since Sharpless initially proposed the concept of click chemistry in 1998 and gradually improved it as a synthetic concept, the core concept of click chemistry is : Synthetic chemistry should be oriented towards molecular function and quickly and reliably complete the chemical synthesis of various molecules through the simple splicing of small units. This theory gives us the possibility of splicing protein sub-molecules and bio-orthogonal chemistry. It has given us another tool. The initial bioorthogonal reaction mainly refers to the coupling reaction, which is used to label, trace, enrich or modify target biomolecules in the complex environment of organisms. After more than ten years. With the development of biotechnology, more than ten bioorthogonal reactions for use in living cells have been discovered or developed. These reactions have played an important role in live cell imaging and biomics analysis research, and have shown great potential. We have combined them. , complete splicing, labeling, tracing, enrichment, etc.”

Although Guan Ruyan is not as powerful as Lu Xiaolu, he is still a doctor in Germany, and his foundation is definitely not bad. He not only understands these cutting-edge technologies, but is also very familiar with them. He talks about them one by one, but he does not talk about the focus of the problem. The focus must be answered by the person who is actually in charge of the topic.

Obviously, the American expert was a little confused, so he was very dissatisfied with Guan Ruyan's answer: "Professor Guan, you didn't answer my question directly. I didn't ask you to answer the history and application of click chemistry and bioorthogonal chemistry theory. , I know you are combining two theories. I want to know how you combine the two theories, what is integrated, and how they are combined organically. I need more details. For example, you told this question to use 2. Sub-function to solve, but this is not enough, you should show the complete process of solving the problem. "

As for the specific details, Guan Ruyan really can't think of them right now. It's like the pattern on a piece of clothing is very beautiful. Someone asked how the pattern on this piece of clothing was made, what artistic inspiration was used for the design of the pattern, and what process was used to draw the pattern. , pattern color, dye formula, etc., these specific details must be known by those who have experienced the entire process.

So to answer these details, Guan Ruyan had no choice but to wait for Lu Xiaolu to come back, but this guy couldn't come back for a long time, and he fainted in the bathroom.

"Sorry, my throat is a little uncomfortable. Let me take a breath." Guan Ruyan took another sip of water to gain some buffering time.

Guan Ruyan waved, and the students at the door came in immediately. Guan Ruyan said in a low voice: "Professor Lu is feeling unwell and just went to the bathroom. I'm worried that he fainted in the bathroom. Go and check."

After arranging the students to go to Lu Xiaolu, Guan Ruyan began to answer the American professor's questions again. He coughed a few times: "My throat is really uncomfortable. It's like a flame burning now. Wait for my assistant to come back and let him answer your question in detail." Question."

He was feeling unwell and his throat was speechless. It would be very impolite to press the question any further, so the American professor temporarily stopped asking questions.

Yang Ping felt very strange. He had just said so many irrelevant words, why didn't he answer his question directly? Confidentiality required? No, there is absolutely no need to keep this secret. He has published everything in the journal Science, and the methods he used were developed based on internationally shared technology. Not only should he, but he is obliged to inform his colleagues.

Yang Ping vaguely felt that there was some special secret behind this. It was definitely not a matter of confidentiality. Could it be that these projects were all completed by Lu Xiaolu and Guan Ruyan didn't know the details? how can that be possible? Yang Ping felt that his idea was ridiculous and disrespectful. Guan Ruyan is a young talent from Nandu Medical University and a candidate for the next outstanding youth. His current scientific research output is very excellent. How could he not be clear about his own topic? It's impossible, there should be other reasons, but Yang Ping can't think of it at the moment.

Academician Xiang frowned and was very unhappy. This kind of meeting is originally a defense meeting. You have to give positive answers to other people's questions. You always go around in circles. Don't your throats hurt when you go around in circles? After going around for a long time, you finally tell people that you have a sore throat. It's obvious. It is disrespectful and inconsistent with the purpose of the meeting.

At this time, the atmosphere of the meeting fell into a brief embarrassment. No one dared to ask questions. It seemed that asking questions was torturing Guan Ruyan. He had already explained that he had a sore throat. If he still asked questions, it would definitely appear extremely inhumane.

what to do? Several professors at Nandu Medical University looked at Principal Rao, hoping that Principal Rao would give an answer. Principal Rao said, "Wait, wait until Lu Xiaolu comes back. Do you have any other assistants to answer for Professor Guan? Professor Guan is unwell." ”

There was an assistant at the door who didn't come in, but it was obvious that he couldn't answer for him. Even Guan Ruyan couldn't answer. This young assistant was just a doctor who had not graduated. How could he answer clearly.

"It's okay, let me answer it!" Guan Ruyan coughed a few times, then took a sip of water, his expression extremely painful.

"Is such that---"

Right at the beginning, there was a violent cough.

"I'm sorry, I'm very sorry -" Guan Ruyan looked like she really couldn't hold on.

Soon, the student who just went to see Lu Xiaolu came back, and Principal Rao asked anxiously: "How is it? How is Professor Lu?"

"The security guard said that Professor Lu almost fainted in the bathroom. The security guard just called a car to take Professor Lu to Fuyi for rehydration."

Principal Rao was confused and very angry, what the hell, you two, no one explained the situation in advance before such an important meeting, and now one has a sore throat and the other has diarrhea, and the diarrhea went directly to the attached one.

Guan Ruyan was also confused now. Lu Xiaolu actually left such an important topic. He was too busy during this critical period and he didn't pay attention to the topic at all. The topic was originally led by Lu Xiaolu. He was too busy and did not familiarize himself with the topic in advance. , I didn’t have much communication with Lu Xiaolu, I was in a hurry, how could I tell you the details? It’s really difficult to get off the ground now, I have never encountered such a thing before.

Now that the assistant is going to the hospital, you can't say that you can't complete the defense meeting without the assistant. If this is the case, wouldn't it be a child's play house? Sore throats and coughs are not that big of a deal. It’s okay to endure it and say a few words.

"Call the school doctor and ask the school doctor to send you a bottle of strep throat hormone and two pills of AstraMe." Principal Rao knows medicine, and he has also encountered this kind of thing when teaching students. It can be easily solved. Isn't it an acute upper respiratory tract infection? If you have a sore throat, the throat hormone will definitely relieve it immediately. For coughs, the effect of Asmi is also good.

This meeting is so important that it cannot be canceled because of this small problem. You must know that some of these experts are from the Imperial Capital, some from the Magic Capital, and some have flown back from the United States. Who doesn't have a slight cold?

Guan Ruyan doesn't want to stop him now, nor does he not stop him. He has completely lost his idea. Lu Xiaolu is not here, so he can't think of any way. Unless he faints on the spot, it is impossible to do such a thing. If not for his psychological quality, he can't do anything. Okay, Guan Ruyan must be sweating all over his forehead now.

"Is Professor Lu okay?" Guan Ruyan asked with concern, trying to delay as much time as possible, and then think about countermeasures.

"I don't know. It's probably dehydration. I'll call the attached office and ask." The student immediately left the venue.

The hormones and Asmeta for the throat were also delivered, as well as warm water. Guan Ruyan had no choice but to take two Asmeta pills, and then use the hormone for the throat. Principal Rao asked: "How is it? Feeling better." Well, I used to use this method when I had a cough and a sore throat in class.

"Let the meeting continue. Professor Guan is not feeling well. I apologize for wasting everyone's time." Principal Rao personally apologized to everyone.

The quality of these professors is really high. When encountering such an episode, everyone did not whisper or show impatience. They were very tolerant of Professor Guan, so the meeting continued as usual.

The other experts were still very considerate, knowing that Guan Ruyan was not feeling well, so they basically asked symbolic questions, which meant that the rest of the meeting was going to go through the motions, but the Americans were not polite at all.

"Professor Guan, I don't know if you didn't hear my question clearly? My question is how do you use the theory of two cutting-edge technologies to achieve your goals, such as the details mentioned in your paper: modular construction based on double-click chemistry strategy Compound library, this is a necessary preparation for your subsequent research.”

Guan Ruyan tried his best to answer: "My paper mentioned active fluorosulfonyl isocyanate, which acts as a bielectrophilic reagent to achieve amidation of carboxylic acid and amine building blocks and SuFEx sequential linking. Both reactions are carried out under simple conditions. It can be carried out, and most substrates can be converted almost quantitatively. N-acylsulfonyl fluoride and N-acylsulfonamide compounds were obtained with excellent yields. This experiment is the basis for our subsequent protein modification and labeling. "

"It's all in these papers. I want to know the details outside of the paper, such as how many microwell plates your initial experiment was performed on, what was the conversion rate? etc."

The Americans ask very detailed questions, and they like to ask questions in a follow-up manner. If you answer one question, they will continue to ask questions along the lines of your answer.

This kind of tracking question confused Guan Ruyan. The experiment was not designed by Guan Ruyan, so how could he know the ideas and details behind it.

Guan Ruyan was stuck for a moment. The Yankee was not ready to give up and was waiting for his answer.

"Just tell him directly. There is nothing confidential about this." Academician Xiang was very dissatisfied with Guan Ruyan's performance.

First you said you had a sore throat and refused to answer the question directly. Now someone else asked you and you were stuck. How could you not know about your own project? Even if the boss handed over the specific work to the following graduate students to complete, the basic design of the experiment was done by yourself. At least the creativity was Yours, so you can definitely answer this question.

Yang Ping thought that Guan Ruyan had hearing loss caused by a cold and did not hear the Yankee's question clearly, so he helped translate: "He wants to know how you completed this pioneering research. You can directly tell him the details of the implementation, such as the number of microwell plates used. , what is the conversion efficiency of the target compound, etc.”

Guan Ruyan flipped through the information on the computer very quickly. The computer was connected to the projector, so the screen switching caused by him frantically flipping through the courseware over and over again.

Where is it? To be honest, today's courseware was all prepared by Lu Xiaolu. He was not familiar with it at all. He was just familiar with it a little before coming here. How could he understand the details?

"Sorry, I really don't remember these specific data accurately. I need to pull up the information and take a look." Guan Ruyan could only say this.

Lu Xiaolu, Lu Xiaolu, you’ve tricked me to death today. I stopped having diarrhea earlier and stopped having diarrhea later, but now I have diarrhea, and it’s as far as Annex 1.

At this time, Principal Rao, Academician Xiang, and Yang Ping all turned their attention to me. Don’t you know? How is it possible? How is it possible that we don’t know such important data? Even if we don’t know, we can at least give a rough idea. And even if we don’t know, we can deduce it from the data in the topic just now. The prediction data in the topic is not inferred using these data and formulas. Came out?

This is off the mark!

"Just give a rough idea. He just wants to know a rough idea, because if some indicators are not up to standard, they cannot be used in your project."

Academician Xiang kindly reminded.

But I don’t know the data, and I don’t know the details inside at all. Just like the director just got the manuscript, he just read it briefly and then went to the stage to speak. Then someone asked such serious questions about the details of the manuscript. How could he tell it? , isn’t this difficult?

It's okay if everyone doesn't urge me, but this urge makes Guan Ruyan even more anxious. The key is that he doesn't know, he doesn't know anything. This is something outside the project, not something in the project plan.

He must not collapse today, Guan Ruyan forcibly stabilized his emotions.