The medical black technology system of the top student

In the rat room.

Wen Yong moved over a large box, which was filled with centrifuge tubes, alcohol, disposable rubber gloves and other consumables that would be used for sampling later.

Shang Peng helped arrange the consumables in the box neatly on the table, and the others began to prepare the mouse anatomy and sampling.

Lu Liang also picked up the alcohol spray bottle and started cleaning the table.

After getting everything ready, Lu Liang put on disposable gloves and then put on a cotton gauze glove used for moving bricks on the construction site.

Although it is difficult to perform some delicate tasks after wearing cotton gauze gloves, doing so can effectively prevent being bitten by mice.

After all, disposable rubber gloves cannot protect mice’s teeth.

If you are bitten by a mouse, even if it only breaks the skin, the rabies vaccine will definitely not be able to escape.

It's different when you wear cotton gauze gloves. Let alone mice, you won't be able to bite them through even if they come.

Lu Liang took out a mouse from the cage. When he pinched the back of the mouse's neck, the mouse was already struggling wildly.

But no matter how hard he struggled, he could no longer escape the fate of dedicating himself to science.

Collect 1 ml of blood from the tail vein, anticoagulate with 3.2% sodium citrate, and centrifuge for 15 minutes at room temperature not exceeding 25°C to prepare platelet plasma samples.

After blood collection, the mice were dissected and sampled. The heart, liver, spleen, lung, kidney, skeletal muscle and other samples were wrapped in tin foil and placed in a liquid nitrogen tank for storage.

The whole process is probably like this.

This process seems simple, but the workload involved is huge. If Lu Liang were to handle all the mice by himself, he would probably be exhausted to death.

Fortunately, this time Song Ke called all the students in his research group over, and even he himself came along.

With the help of Wen Yong, Shang Peng and others, things will be much simpler.

Blood collection, dissection, sampling, sample packaging, and preservation.

As the mouse sampling work began, everyone began to get busy.

The entire rat room is like a running assembly line, and everyone has a clear division of labor.

One after another, the mice embarked on the fate of dedicating themselves to science.

Finally, after finishing the blood collection of the last mouse in the mouse cage, Lu Liang breathed a long sigh of relief and rubbed his sore neck.

He took out his cell phone from his pocket and saw that it was already half past eleven.

They started at eight o'clock in the morning, which meant that for almost four hours, they didn't stop for a moment.

Then he raised his head and glanced at the people in the rat room. Everyone had tired faces now, and they were obviously exhausted.

Sampling work seems easy and does not require much physical strength, but the boring and repetitive work like an assembly line does make people easily mentally exhausted.

Lu Liang said: "After everyone has collected samples, I'll treat everyone to a meal."

Treating guests to dinner after collecting samples can be regarded as an old and tacit rule among major research groups.

After all, people put down their work and went all the way to help you take samples. Not to mention labor fees and the like, you should always treat others to a meal.

"Okay, thank you Brother Lu."

"Everyone, please work harder to clean up the rat room and go eat."

As soon as they heard that they were going to eat, everyone suddenly became energetic, and the movements of each hand could not help but speed up a lot.

Lu Liang said to Wen Yong: "Brother Yong, I will trouble you to take everyone to a place to eat later. I will transfer the meal money to you when the time comes."

Wen Yong was a little surprised: "Aren't you going?"

Lu Liang said: "I still have to go back to the laboratory to process the samples."

Wen Yong hesitated and nodded: "Okay, then after we finish eating, we will come over to help you process the samples."

"That would be troublesome."

Finally, before 12 noon, all the mice were completely sampled and the rat room was cleaned.

After completing the sampling work, Wen Yong took people to eat, while Lu Liang took the samples and went straight to the laboratory.

After storing all the samples in a -80°C refrigerator, Lu Liang began to list the various indicators he needed to detect in an orderly manner.

Plasma testing uses a fully automatic coagulation instrument, and the detection index is activated partial thromboplastin time.

Kit detects coagulation factor VIII activity and coagulation factor VIII inhibitors

Use PCR or qPCR to detect the distribution and transcription of coagulation factor IX and coagulation factor X in the genome of various mouse tissues using PCR or qPCR.

After listing all the indicators that needed to be tested, Lu Liang once again devoted himself to the work of sample testing.

Late at night, in front of the computer.

Before treatment with emicizumab, the activated partial thromboplastin time of mice was 112.00±21.91s.

After treatment with emicizumab, the activated partial thromboplastin time of mice was 24.63±1.71s.

The mice clotted blood significantly faster.

Coagulation factor VIII activity in mice before emicizumab treatment.

Looking at the data results on the computer screen, Lu Liang rubbed his cheeks, then lay on the chair and let out a long sigh of relief.

The test data came out much faster than he expected.

With the help of Wen Yong, Shang Peng and others, several people adopted a 24-hour shift strategy and took turns testing the samples.

It is precisely because of this that Lu Liang was able to obtain all the data in such a short period of time.

Judging from the current data, the final result was the same as Lu Liang expected.

Imicizumab is effective in the treatment of hemophilia.

Lu Liang opened his email and sent a copy of the detected data to Ji Hong and Song Ke respectively.

So, now all that is left is the Phase III clinical trials of the drug.

Early in the morning, in Ji Hong's office.

"Genius, this is the way of thinking of a genius. Lao Ji, your student is really a fucking genius!"

Ji Hong sat on the chair and turned his head slightly to prevent Song Ke's flying spittle from splashing on his face.

Now Song Ke's face was flushed, and he was so excited that he could not speak coherently.

After reading the data Lu Liang sent him last night, Song Ke was so excited that he didn't sleep all night.

Early the next morning, he ran to Ji Hong's office to vent his feelings.

"Old Song, calm down, calm down." Ji Hong slowly unscrewed the lid of his thermos cup and took a sip of water.

He was already accustomed to this result.

"No, Lao Ji, I can't calm down, how do you ask me to calm down!"

It can be said that Song Ke has been immersed in the research of hemophilia for most of his life. Now that he sees the emergence of a real drug to solve hemophilia, how could Song Ke not be excited?

"Drink water, drink water, Lao Song."

Ji Hong took a disposable cup and made a glass of ice water and handed it to Song Ke.

Finally, after drinking several glasses of ice water, Song Ke finally calmed down a little.

Ji Hong said: "Old Song, I am now thinking about where to submit Xiao Lu's article."

"Don't worry, this must be an official issue of Nature or Science. It is a pity that this epoch-making drug in the field of hemophilia is not included in an official issue!"

Song Ke replied without thinking.

Ji Hong nodded. He agreed with Song Ke's statement.

Lu Liang's monoclonal antibody drug not only provides a new idea for the treatment of hemophilia, but also proves that this idea is feasible.

"So, what I am struggling with is which of the two should I vote for." Ji Hong said.

"Uh" Song Ke also became entangled. In fact, whether it is Nature or Science, the influence of the official journals is completely comparable.

There is no problem in submitting to either of these two journals.

"It's better to be Nature."

After Ji Hong struggled with Xiao Hui, he made a decisive decision.

Within the country, Nature’s reputation still needs to be louder.

After finalizing the journal submission, Ji Hong looked at Song Ke: "By the way, Lao Song, you have worked hard during this period. If it weren't for your help, Xiao Lu's experimental data would not have come out so quickly."

Song Ke waved his hand: "What a big deal, we are all in a big team, we are just busy, nothing important. If there is nothing else, I will leave first."

Ji Hong thought for a moment: "Don't leave in a hurry, Lao Song, haven't you ever thought about putting your name on the article?"

"Hum, if Mr. Lu is willing to show me the data, I will be very satisfied. For things like the second author, I won't be shameless enough to go to him."

Song Ke knew very well that this article was of great significance to the research field of hemophilia.

He really wanted to leave his name on this article, but he really couldn't get that old face to ask for it.

Ji Hong said: "I'm not talking about the second author or anything, I'm talking about communication."

Hearing Ji Hong's words, Song Ke was a little stunned: "Classmate Lu's mentor is you, Lao Ji. Don't you want me as the corresponding author, Lao Ji?"

Ji Hong rolled his eyes: "Old Song, are you confused? We are communicating together, communicating together!"

Song Ke just reacted at this time. He rubbed his hands and looked a little awkward: "I'm sorry."

"Then you don't want it, right?"

"I want it!"

Song Ke hurriedly spoke, he would not refuse it until his head was kicked by a donkey. Even if it is a communication, the gold content is definitely not low!

Song Ke was elated at the moment, but he soon hesitated: "Do you agree with this matter, Mr. Lu?"

Ji Hong thought for a moment and then replied: "It's okay. I will go to Xiao Lu to discuss this matter. It shouldn't be a big problem."

"By the way, Lao Ji, is Mr. Lu very interested in the research direction of hemophilia?"

Song Ke seemed to have thought of something, and he suddenly changed the topic and asked.

"I'm probably not interested in the direction of hemophilia. What's wrong, Lao Song?" Ji Hong frowned and asked.

"Nothing, I'm just asking. In addition, which research direction is Mr. Lu interested in?"

Song Ke asked tentatively.

Ji Hong's expression immediately became wary: "Why do you ask this?"

"Old Ji, I don't want to think about it. Isn't Mr. Lu still an undergraduate now? He might have new ideas when he goes to graduate school in the future, so I changed the research direction in advance to a research direction he is interested in."

Ji Hong's eyes widened: "Hey, hey, Lao Song, what do you mean? I've already communicated with you, and you still want to hit my student's attention.