Rebirth of the God-level Xueba

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Yang Rui slept in the ion channel laboratory for several days in a row.

)

It wasn't that he wanted to overdraw his physical strength to fight from the beginning, but comrades Su Xiankai, Fan Zhenlong, and Wang Sisheng had high fighting spirits, so he had to reluctantly work overtime.

In fact, if he entered the middle of the experiment, the three people working overtime might not necessarily affect him. The scientific research apes were exhausted and the breeders traveled around the world to give speeches too much. However, the early stage of the experiment was different. There were so many things Yang Rui needed to confirm that the three of them had so many questions that Yang Rui couldn't hide.

The job of the project leader is like this. If there are no problems with the big cows and the little scientific research dogs under him, and they are all working step by step, then he can work step by step, and even take vacations step by step, just like the rancher. Cowboys are powerful, and when there is nothing around, you can enjoy the fresh air, the delicious steak, and the fatness of cowgirls, cowboys, or sheep.

However, the weather is good, the cowboys are strong, and there are too few situations where there is nothing around.

Especially for a new ranch, it was a rush of business. Just confirming the purchase of reagents, Yang Rui was busy for an afternoon.

If the G protein-coupled receptor group runs smoothly, Yang Rui can completely ignore the purchase of reagents or something. It is just a routine laboratory life, just like what brand of gloves to buy and what type of gloves. It's as easy as pulling paper.

But at the beginning stage, Yang Rui really couldn't take these small problems lightly.

The simplest purchase of reagents also pays attention to compatibility. For example, whether to buy domestic reagents or imported reagents, it seems quite easy to say. If you have money, you can buy imported ones, and if you have no money, you can buy domestic ones.

For scientific research dogs who graduate in two or three years, maybe the answer is true, but from the perspective of the project leader, the problem is completely different.

What Yang Rui needs to determine at the beginning is actually the reagents needed for the entire experiment cycle. Otherwise, if you use imported reagents in the early stage and domestic reagents in the later stage, and then compare the two, the result curve must be messy. In this kind of high-standard international scientific research competition, the articles are all aimed at s, and the pictures in it are all to be read and even repeated experiments by people, and a good data will be ruined if it is messy.

Furthermore, the purchase of reagents is not limited to the difference between imported and domestically produced reagents. Yang Rui’s final answer on paper must not only determine which country it is from, but also which company’s factory, or even a certain batch of reagents. .

The purity of reagents can be selected from superior grade pure, analytical pure, chemical pure, chromatographic pure, etc.

Respectively correspond to the amount of impurities. However, for some reagents, no matter how high the standard is, it is still impossible to completely eliminate impurities. For those who do experiments, this is a very annoying thing. You do not have the conditions. How to do the experiment?

It's a pity that it doesn't mean that you can talk about scientific research if you don't have the actual conditions. The correct way is to ensure the consistency of impurities. The impurities of the same batch of reagents in the same factory can usually be guaranteed to be consistent. In this way, through comparison And calculation, the influence of impurities can be eliminated to a large extent.

By extending the same method, two conclusions can be drawn. First, even if it is a high-purity experimental reagent, it is better to buy a batch, because you don't know if the other factory will produce a batch of substandard reagents out of the blue, and you will encounter it. It is imprudent and unprofessional to put your own years and millions of dollars on the prudence and professionalism of others.

Not to mention, scientific research is originally doing things with small probability.

The second conclusion is that relatively high-purity reagents can be used to do relatively demanding research, as long as the data is processed in the later stage-this is a completely compliant processing, and the results are similar to experiments made with medium-high purity reagents . Chinese laboratories have always done this, the main reason is... of course because of being poor.

In addition, reagents are not as pure as possible.

The first question to face is naturally whether the wallet can bear it. If the wallet can bear it-in extreme cases, there are actually examples of not buying high-purity reagents. For example, for materials, they often like to buy domestic reagents. Because domestic reagents often have Inexplicable impurities can occasionally bring surprises to people.

There are many examples of so-called impurity crimes in the history of science, and they became popular in late 2016 and early 2017.

Secondly, having a big purse is not about buying reagents and looking at expensive ones.

You can’t buy analytically pure concentrated sulfuric acid used to treat waste liquid. Even if you are rich in oil, dismantling the analytically pure small packaging bottle and pouring it into the waste liquid bucket is a bit too hard.

Totally thankless.

Besides, there are no really rich people who do scientific research on the earth. Take the biological laboratory in 1986 as an example. One gram of interferon worth 50 million US dollars, you can say how many tons you want.

G protein-coupled receptors are originally a money-burning project. The start of 6 million US dollars is enough, but it must not be wasted at will, especially in the early stage, and the establishment of standards must be more careful.

Just because the fog of war is turned on, you can't immediately come to a double base.

Many of the reagents used in the early stage will be used all the way to the end of the project. If the project will last for three to five years, or even five or eight years, it is impossible to suddenly switch to cheap reagents in the fourth and seventh years. The value of the previous experimental data will be greatly reduced, so instead of gnashing your teeth at that time, you must establish your own standards in the early stage.

This is actually a challenge for Yang Rui.

How to plan for the start, no one taught me, and there is no fixed number, the world is changing too fast, to some extent, there must be some predictions, this kind of prediction, not only to predict the time to do the project, but also the expected In terms of funding, it is also necessary to predict the status of the opponent and the trend of the scientific research community.

Of course, the most important thing is experience.

If he was in developed countries such as Europe and the United States, Yang Rui could still try to consult scholars from other top laboratories, but unfortunately, there is no corresponding condition in China.

It is Professor Cai and others, although they have made world-class results, they have not established a world-class laboratory, and have obtained world-class results while carrying out world-class projects.

All in all, since the artificial synthesis of bovine insulin in China, there has been no such targeted large-scale biological research and development. In the past 20 to 30 years, there have been basically no scientific researches in the field of biology, showing their chariots and horses, preparing for war, and then participating in positive scientific research competitions at the international level. Generally speaking, they are almost all ambush warfare special warfare or special forces behind enemy lines.

Back in the day, when the Institute of Biochemistry of the Chinese Academy of Sciences, the Department of Chemistry of Peking University, and the Institute of Organic Chemistry of the Chinese Academy of Sciences assembled heavy troops, organized the distribution of jackets, led the mobilization before the war, and had a scalpel in hand, when they opened tablets of synthetic bovine insulin, "se" and " "nature" is posted one after another. Although it is only an American laboratory and a German laboratory that are fighting with the power of the whole country, in a specific scientific research field, this is indeed the highest level of confrontation. It shocked the world. Maybe not, shocking China is indeed done.

However, that was also 30 years ago.

Therefore, Yang Rui has no place to ask for advice.

However, on the bright side, this also made Yang Rui have no competitors, and less expert guidance.

"Anyway, it's my own money, I'll pay for my own tuition..." Yang Rui gritted his teeth and signed various documents and projects.

The U.S. dollar account stored in the People's Bank of China has also declined rapidly, and the distressed branch business managers have called to inquire.

100,000.

$200,000.

$500,000.

1,000,000.

$2 million.

By the time the preparatory work was completed and the instruments and consumables to be used in the early stage were ordered, the $2.7 million had already been paid out.

Looking at the figures announced inside the laboratory, Su Xiankai's head felt numb at the time, and cold sweat rolled down his back in an instant.

"It's a good thing you didn't listen to me at the time." Su Xiankai followed him from beginning to end, knowing that Yang Rui didn't choose particularly expensive products, and basically looked for them in the mid-to-lower range. It's not that you can't be more frugal, but if you continue to be frugal, you can only do follow-up experiments, and you are not qualified to participate in scientific research competitions.

However, with such a standard, a general selection, unexpectedly reached 2.7 million US dollars, almost three times the amount Su Xiankai wanted, and Su Xiankai was also frightened for a while.

There is no turning back when the project starts, and $1 million has been spent. If you don’t see a sound, you won’t be able to get it back.

Yang Rui didn't feel lucky either, he just said: "Needless to say that you succeed once, the rest of the funds can kill people, maybe there will be one or two seven-figure figures left..."

"Director Yang, please don't provoke us, we haven't even found the door yet." Fan Zhenlong couldn't help laughing. Recently, he and Wang Sisheng were asked to practice just a purified g protein, let alone the whole set of experiments.

Su Xiankai, a skilled worker who has already purified a set of g protein, is not confident at all, and said: "If you only make rhodopsin protein, then if there is one more success, I already have a feeling here, but you definitely can't do it if you ask for it." So low."

Yang Rui rolled his eyelids and said, "Bovine rhodopsin protein is an experimental material, and it hasn't entered the experimental procedure yet. Lao Fan, what about your side? Is the detergent good?"

Using detergent can increase the solubility of g protein, which is currently the best way to purify g protein, and it is still a secret book in high-end laboratories, and it has not been widely disseminated.

But Fan Zhenlong still shook his head and said: "It's easier to use, but we haven't found the trick to extract the receptors from the cell membrane. Old Su can't figure it out either."

Yang Rui naturally looked at Su Xiankai.

Su Xiankai blushed, and said: "Old Fan and the others chose to purify gt, and I have never touched it before. I will start over again, and I am not sure about the various dosages. Actually, I don't have any tricks, I just do it too much. This is done. It took a year to purify it."

There are many types of g-protein and g-protein coupled receptors, and a certain amount is required to make a systematic comparison. Therefore, although purification is for Fan Zhenlong and others to practice, it is also one of the necessary conditions in the early stage of the experiment. one.

But if you have to wait another year or a half before one more purified protein is produced, the laboratory can't afford to wait.

Yang Rui couldn't help lowering his head in thought.

Using detergent to purify g protein, this secret is also what he read from the book, and he has never done it in the field. Moreover, based on his scum experimental level, there is no specific experimental guidance.

In any case, Fan Zhenlong and Mavericks' experimental level is also better than his. However, their future success does not depend on their experimental level. In terms of experimental level alone, the three of them may not be as good as Gu Qiang.

Thinking of this, Yang Rui couldn't help but patted his forehead, and said: "In this way, I will add another farmer for you... No, I will add an experimental assistant, Gu Qiang from the genetic engineering laboratory. a bit."

Learning also needs help. Since the speed of idle mining is slow, it is better to add a farmer.

Anyway, the genetic engineering laboratory is also under his name, and they are all public laboratories, so it is very convenient to second or something.

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