Rebirth of the God-level Xueba

"Director Yang, what's the situation here?" Pang Jiancheng asked Yang Rui with a smile, thinking to himself: The acting skills are quite good, and Peking University is really a hidden dragon and a crouching tiger.

No one thought that there would be new research results coming out, and Pang Jiancheng naturally didn't expect it. He only thought that this place was arranged by Yang Rui, so he made him a fan.

Instead, a few foreigners, listening to the "three-dimensional structure" that the translator said, whispered.

Yang Rui hesitated for a moment, coughed twice, and said, "There seems to be good news coming out of our laboratory, please wait for a while."

After speaking, Yang Rui followed the student back to the laboratory, and directly asked for the photos taken.

Gu Qiang and a group of people gathered around the electron microscope with different expressions.

"I wanted to be idle, so I just took a few photos. However, I used the materials left over from the second phase of the repeated experiment. I don't know if it will interfere with the experimental results." Gu Qiang was a little proud, He explained to Yang Rui worriedly.

The boss of the laboratory holds all the power in the laboratory, and the results made by the subordinates must be approved by the boss.

Otherwise, if you encounter the worst laboratory leader who refuses to admit the experimental results of his subordinates, or even denounces them as fraudulent, then his subordinates can only swallow tears in their stomachs—going to other laboratories to do repeated experiments is a matter of fact Simple is difficult to do, otherwise, Yang Rui would not have mobilized everyone that day, and used a lot of experiments and resources to do the second phase of repeated experiments. It was still in the case of a laboratory, and it would be even more difficult to change laboratories. .

Not only that, but the researchers who leave the laboratory of the big brother-level scholar will encounter many other problems. It cannot prove that you did not cheat this time, nor can it prove that you did not cheat last time.

Galileo's experiment of two small balls falling at the same time was suspected of being falsified. The correct theory does not mean that the experiment is correct.

On the contrary, there are more scholars who have ruined their reputation and left the scientific research circle sadly.

Therefore, no matter what achievements Gu Qiang has made, his first and best choice will always be to get Yang Rui's approval, and then see how much credit Yang Rui can give him.

After the streptomycin incident, the value of specific executors in academia has become quite clear.

"Spread out and do your own thing." Xu Zhengping expelled everyone around him before Yang Rui began to watch the movie.

Before the official publication of the paper, the electron microscope photos are first-hand evidence and first-hand information, which must not be shown to others.

Biologists have always criticized Watson and Crick for sharing the dna double helix structure of the Nobel Prize. One of the reasons is that they suspected that they had peeked at Rosalind Franklin's X-ray crystal diffraction pattern-of course, they must have seen it Yes, the key to the question is whether it was a peek, because Ms. Franklin died young, and the arguments in it are irrefutable.

However, the crux of the problem is actually a photo.

However, what kind of picture is the X-ray crystal diffraction picture of DNA taken by Rosalind? White flowers on a black background, two crossed black reflective strips, thrown on the street, the kind that no one is willing to take a second look at.

Watson later wrote that he was stunned and heart-pounding at first glance, because only the helical structure would show up on a photograph as two crossed black reflective bars.

No matter what the photo Gu Qiang made, it should not be made public in the laboratory before the paper is published.

All the researchers needed was a reminder from Xu Zhengping, and all the researchers came to their senses and returned to their positions one by one. Only Gu Qiang was left nervously looking at Yang Rui.

Yang Rui nodded to Gu Qiang, then took out the photo and read it carefully.

Compared with others, Yang Rui has always had an advantage in reading images and other aspects.

He does not read the specific image of the photograph, but only sees whether the image conforms to the theory of three-dimensional structure, which is what all people who look at the image need to do.

Chapters such as why do we learn the eight forms of fractures in school are used to check whether there are corresponding elements on the image. If there are, it proves that there is a fracture, and if not, it proves that there is no.

However, scholars in the 1980s knew little about the three-dimensional structure of g protein-coupled receptors, which gave Yang Rui a comparative advantage.

He only looked at it for a while, then put the image back into the bag, and then looked at Gu Qiang with scrutiny.

"Director Yang..." Gu Qiang shouted with a guilty conscience.

"Have you recorded the experiment?"

"I've done it." Gu Qiang quickly took it out. This is a good habit, and it is also the place where the ion channel laboratory is better than other laboratories. Even if it is a random experiment, you must make experimental records, and you must write it down while doing it. .

Experiment records are useless in most cases, but when you discover something, especially a world-class discovery, your only regret is that the experiment records are not detailed enough!

"It would be fine if I had..." is often said by those who have not received systematic training.

The purpose of systematic training is to prepare researchers well and seize every hard-won opportunity. After all, the number of times a person steps on dog shit in a lifetime is limited, except for Gu Qiang.

Yang Rui glanced at Gu Qiang again, read the experiment record, and said: "There is no problem for now, but I still want to see the repeat experiment."

"Of course." After Gu Qiang finished speaking, he whispered: "In this case, will the three-dimensional structure be made?"

Yang Rui hesitated for a moment, then said, "Basically."

"Yeah!" Gu Qiang shouted in a low voice.

"I still need to perfect the remaining parts and draw the three-dimensional structure, but that's basically it, the rest is rough work." Yang Rui said with some emotion.

Regarding the third phase of experiments, that is, the three-dimensional structure of G protein-coupled receptors, the most important and main requirement is to find the activation mode of small extracellular ligands and G protein-coupled receptors.

To put it simply, as a protein, g protein-coupled receptors are invisible to the naked eye, but they are very large at the molecular level. It is like a molecular building, with people and cars coming in and out all the time.

These people or cars coming in and out are extracellular ligands.

For example, how does the human sense of smell arise?

The outside air contains various molecules. When they enter the human nose, some of the molecules that can be distinguished by humans will come into contact with a certain g protein-coupled receptor in the human body. Pedestrians in the g-protein coupled receptor building.

When pedestrians enter the building, the building sends a signal to the brain, which searches for information and tells humans what you just smelled.

To delineate the three-dimensional structure of G protein-coupled receptors is to describe the appearance of this building, and the most difficult thing is not the outline - the outline of the huge G protein-coupled receptor is still easy to make, the most difficult and the most difficult The main and most important part is how it accepts pedestrians and vehicles.

Specifically, look at the location and shape of the various openings on the G protein-coupled receptor molecule.

In other words, what human beings want to know is where is the elevator of this G-protein coupled receptor building, where is the garage door, where is the main entrance, side door and back door, and whether there are overpasses and rooftops.

Using an instrument to scan g-protein coupled receptors is like scanning a building with a laser. They don't really see everything in the building, but need to rely on various feedbacks to get answers.

It's like shining a laser on the door of a building. If the door is closed, the laser will give a reflection and get the wrong answer that there is no door here.

The scanning of G protein-coupled receptors is more complicated, because the protein is mobile, and it will show the necessary three-dimensional structure only when it has a relationship with the extracellular ligand, otherwise, it is a closed protein.

To put it bluntly, g protein-coupled receptors often exchange with the outside world, but it is a test of luck to capture such a momentary picture.

Historically, it took about 4 years for Kobe Karl to burst out of character and capture the desired scene—the moment when a certain extracellular ligand touches a g protein-coupled receptor.

Gu Qiang spent about four months doing relevant research.

The triumph of shit luck over hard work has happened countless times in history, and it won't be the last.

But for Yang Rui, this timing was too coincidental.

"You continue to do repeated experiments, and write down all the experimental records you can think of." It is impossible for Yang Rui to give up this achievement. The next time he takes the photo he wants, who knows what century it will be.

However, Yang Rui cannot let these achievements be exposed to the Swedes unprepared.

No scholar can resist the temptation of the Nobel Prize.

"Who can take over and repeat the experiment?" Yang Rui raised his voice and asked.

Several researchers looked at each other, and finally Su Xiankai stood up.

Yang Rui nodded and said, "Now separate the experiment of the second stage from the experiment of the third stage. The electron microscope is not allowed to move. Tian Bing, you will assist Gu Qiang in making records."

It is a normal procedure to complete the records first and then repeat the laboratory.

After a little fuss in the laboratory, Yang Rui took another look at the electron microscope, and walked out despite some hesitation.

"Everyone, we have just completed a new round of repeated experiments, which prove that we have obtained the complex of the N-terminal ligand domain of the G protein-coupled receptor and the ligand binding, and its crystal structure has been completed." Yang Rui In an announcing tone, I faced a group of Swedes who were stuck out by hot pot.

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