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Chapter 143 Cultivating dinosaur embryos

"Start simulating chromosome structure...eliminate 520,000 results."

"Start running histone binding simulations... 490,000 results eliminated."

"Start simulating the construction of a cell nucleus..." After layer by layer of screening and elimination, Du Xingyu obtained more than a hundred genetic maps that could be constructed into a dinosaur cell nucleus.

There are still flaws in these maps, and he needs to eliminate and correct them one by one.

After another week of work, Du Xingyu finally obtained a complete genetic map of Tyrannosaurus rex.

Relying on this genetic map, he can synthesize genomic DNA, combine it with histones, and put it into the nucleus of the cell to form a nucleus containing the genetic genes of Tyrannosaurus rex.

This has only completed ten percent of the process of cloning Tyrannosaurus rex. Next, he needs to transplant this cell nucleus into cells to construct "dinosaur cells" and induce them into pluripotent stem cells.

Some of the biotechnologies involved have exceeded the scope of current human technology. Du Xingyu needs to rebuild research projects and overcome them one by one.

………

One semester has passed in a flash, and it is mid-January, the school's winter vacation.

"Du Xingyu, I'm leaving now. See you next year." Bao Xian had already packed up his gifts and was preparing to go home for the New Year.

"Bye." Du Xingyu said goodbye to him.

In the laboratory, the team members also went home for the holidays.

"Du Xingyu, aren't you going home?" Du Xingyu's mentor Su Yutang asked curiously.

"I want to continue researching this topic. There are fewer people in the laboratory during the holidays, so I can use it more." Du Xingyu explained.

As a graduate student, he doesn't have much time to actually use laboratory equipment. Many of the equipment are shared by several people. Du Xingyu wants to take advantage of the Chinese New Year to cultivate Tyrannosaurus rex stem cells, so that he can further cultivate Tyrannosaurus rex embryos. .

Besides, Du Xingyu doesn't have many family members in this world.

"Academic research is important, and life is equally important." Su Yutang held his granddaughter Su Ziyun with one hand, put the key in his hand with the other hand, and said: "Your paper on gene sequencing is very good. You can try to submit it to " "Cell" submission!"

"Well, I'll do it some time." Du Xingyu said calmly.

Su Yutang glanced at him in surprise. "Cell" is one of the most influential journals in life sciences, alongside "Nature" and "Science".

No matter who I tell him that his paper is qualified to be submitted to "Cell", I'm afraid they will be so excited that they can't sleep, right?

Isn't this student a little too calm? This is like telling an ordinary person that he won 100 million in the lottery, but this person didn't react at all!

"Do it well!" Su Yutang said.

"Goodbye, brother Xingyu." Su Ziyun said goodbye politely. She waved and said, "You can definitely develop the dinosaur burger!"

Du Xingyu: "..."

Du Xingyu finally had a lot of time to use the laboratory's high-end equipment. It was much more difficult to build a Tyrannosaurus rex cell nucleus in person than on a technological simulator.

"Hello everyone, this is the Creator VLOG. Today is the 9th video." Du Xingyu still insists on updating his video log, although the number of fans has barely reached 100.

"In the previous stage, I have built the nucleus of the Tyrannosaurus rex, and today I will transplant it into the recipient cells to grow real dinosaur cells."

"I won't show you the operation process... After several failures, I finally got a mature dinosaur cell."

"This cell needs to continue to be cultivated, divided, and then induced to become a stem cell."

A day passed after Du Xingyu posted the video, and there were only a few comments.

0:51: " @Creator VLOG, the author should change the subject. No one will watch this kind of video."

FIHJJ: "I haven't seen it in a long time. Is this person still cloning dinosaurs?"

Tomato Juice: "It's been almost half a year, why haven't you cloned it yet?"

Jiang Ye: "Hey, I actually met a hardcore author. I am also a student in the Department of Biology. I think the author is very powerful. Let me go and watch your previous video!"

On New Year's Eve and New Year's Eve, thousands of houses are lit up with lights.

The whole country was enveloped in the joy of family harmony, and Du Xingyu was still researching in the laboratory.

He has cultivated dozens of generations of Tyrannosaurus rex pluripotent stem cells, constantly inducing, screening and confirming them, hoping to obtain a stem cell that can be used to cultivate embryos.

Thanks to the technology simulator, he saved a lot of trial and error costs. If Du Xingyu is really allowed to try it slowly by himself, I'm afraid it will be difficult to complete in his lifetime!

The technological simulator can eliminate countless error possibilities in advance and generate simulation results in an instant from a test process that would have taken years or even hundreds of years!

Under a biological microscope, Du Xingyu finally discovered a dinosaur stem cell that met the requirements in an in vitro cell culture dish.

"Great!" Du Xingyu was overjoyed, "Finally we can breed dinosaur embryos!"

It is also extremely difficult to induce stem cells to develop into embryos in vitro. But Du Xingyu was not worried at all. He had even cultivated an individual Tyrannosaurus rex on the technology simulator.

Just because of the limitations of the actual experimental process, it has not been completed yet.

"If everything goes well, I will probably be able to complete it in three months." Du Xingyu thought to himself.

He picked up his phone and saw New Year greetings from friends on the message bar. Du Xingyu edited the message and sent it: "Happy New Year!"

Winter break is over and students are back to school.

Bao Xian rushed to the laboratory immediately and shouted: "Du Xingyu, Du Xingyu! Where are the dinosaur embryos you cultivated?"

"Don't tease me. I still have a week off. I heard what you said and I'll be here right away!"

In a recent chat, he learned that Du Xingyu was already cultivating Tyrannosaurus rex embryos in vitro.

"In the embryo breeding room." Du Xingyu smiled.

"Let me see!" Bao Xian didn't care about anything else and immediately went over to watch.

In the embryo breeding room, dozens of tiny embryos are taking shape. Du Xingyu used biological culture fluid to simulate the conditions of the mother body and cultivated it.

Because the dinosaurs have long been extinct, it is now difficult to find suitable surrogate hosts. Du Xingyu originally wanted to use birds as surrogate hosts for dinosaur embryos, but he found that the eggs of Tyrannosaurus rex were too big, and only ostriches in the world could barely lay them out.

However, what vertebrates express during the development process will be affected by the genes and proteins of the mother. Du Xingyu simulated it countless times using technological simulators, but most of them failed.

Only a few times, a simulated surrogate ostrich gave birth to a half-bird, half-dinosaur monster. Such little monsters would die within minutes of breaking out of their shells.

Therefore, after comprehensive consideration, Du Xingyu chose to completely simulate the situation of the dinosaur mother body to provide a development environment for the Tyrannosaurus rex embryo.

"Is this true?" Bao Xian found it hard to believe that these embryos were Tyrannosaurus rex embryos. These embryos had just begun to develop. It would take dozens of days to develop into a Tyrannosaurus rex egg.

"Of course it's true," Du Xingyu said, "But I predict that out of dozens of samples, only three or five will eventually develop into eggs. Only one or two will be able to hatch successfully."

Du Xingyu was conservative. According to the simulation results on the technology simulator, the survival rate of these embryos should be higher.

"Damn, don't tease me. If this is a Tyrannosaurus rex embryo, then you are a big deal!" Bao Xian said, "You have done something that scientists around the world have not done!"

"I'm not sure yet," Du Xingyu said.

"No, I still don't believe it!" Bao Xian shook his head repeatedly, "Even if you find the genetic map of Tyrannosaurus rex, it's impossible to encode its nucleus and cultivate stem cells in such a short time... You won't make a mistake even once. Going out?"

"How much work is there, how many world-wide problems can you complete alone?"

"Is this another animal embryo? Tell me, did you secretly change the subject?"

"Believe it or not." Du Xingyu couldn't explain it. You couldn't tell him that you carry a black technology plug-in with you, right?

"Are these really Tyrannosaurus rex embryos?" Not only Bao Xian, but other members of the laboratory were also puzzled.

Pang Yan said doubtfully: "It doesn't make sense, this..."

"Junior brother, is this an embryo of another animal? It looks like some kind of bird." A senior sister said.

"Du Xingyu, you teased us when we first arrived." Yang Mu said, "I don't believe it can really breed a Tyrannosaurus rex!"

Du Xingyu said helplessly: "The embryo has just started to develop now...it will take a while to see it."

"Your Tyrannosaurus rex genetic map cannot be proven to be true at all! There are no living Tyrannosaurus rex in the world!" someone said.

"Impossible, impossible!" said a fellow doctor named Zhu Lianbo, "If this is a dinosaur embryo, I can believe it!"

"Don't believe me." Du Xingyu said, "Let's do this. If I really cultivate a dinosaur embryo, you can treat me to a meal. If I fail, I will treat everyone to a meal."

"Okay!" Yang Mu said, "That's it!"

"Okay, then you have to cultivate it well!" Pang Yan, Zhu Lianbo and others said.

Du Xingyu's cultivation of dinosaur embryos not only surprised and puzzled everyone in the laboratory, but even Su Yutang came to see it in person.

He carefully read some of Du Xingyu's experimental records and said very seriously: "Du Xingyu, no matter whether this experiment can be successful or not, you have already said it has been successful!"

"Encoding your own genome and inducing stem cells to cultivate embryos. This technology alone is enough to alarm the entire biological world!"

"From now on, your project is the top priority project in our laboratory, and all experimental projects will make way for it!"

"I hope you can come up with more research results!"

"Thank you, teacher!" Du Xingyu thanked him.

Su Yutang's explanation made the entire laboratory members even more surprised. Is Du Xingyu really that powerful? The teacher actually attaches great importance to Du Xingyu's project. Is this cloning of Tyrannosaurus rex really the mastermind?

Thanks for the reward from I Let You Fei and Yan Hongxue.

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