Rebirth of the God-level Xueba

"Old Su, we don't have enough protein here." Fan Zhenlong came around from the side of the experiment table, called out, and then retreated to get busy again.

Su Xiankai snorted twice and complained, "Why are you running out again?"

"I can't help it, I can't always produce results." Fan Zhenlong said annoyedly: "The amount of protein used here is going to exceed the plan."

Su Xiankai's eyebrows twitched twice, and he said, "I'm not even sure I can make the planned amount. What should I do if you exceed the plan?"

Fan Zhenlong said helplessly: "Of course we don't want to exceed the plan... It can only be said that our early thinking was too simple, and the three-dimensional structure of this thing is much more difficult than expected."

"It's really difficult." Gu Qiang was very reluctant to admit it, but he still said: "Our original plan may have to be completely revised..."

"No need to modify." Yang Rui kept scanning the documents, and said, "The newly ordered equipment will arrive soon. Besides, Lao Su, I will add two more people for you."

At the beginning of planning, Yang Rui knew that the budget would be greatly overrun, and he made various preparations.

With 6 million US dollars, he only spends half of it carefully, and the rest is for the reserve team. Besides, he still has a large sum of RMB that has not yet been used. The funds for the ion channel laboratory, plus the funds allocated by the 863 plan, It's not a problem to make up three million yuan.

If it wasn't enough, he estimated that he could get another sum of money from Peking University. In theory, he could raise a considerable sum of money from domestic biomedical and animal husbandry central enterprises and even ministries and commissions.

This is the advantage of raising your reputation. As long as the project is successful, these donations are all for nothing.

As for the failure of the project, the loss is the prestige itself.

In fact, Yang Rui didn't know how much money was needed to make the desired result, but he knew how much the foreigner used, so he had a considerable estimate of the difficulty of each link.

As soon as Su Xiankai turned his head, he knew that Yang Rui was well prepared, which made him a little embarrassed.

Yang Rui saw it, and made Su Xiankai embarrassed for a moment, and said: "Our plan is based on the comparison of foreign first-class laboratories. Looking at it now, we have not yet reached the level of foreign first-class laboratories. It doesn't matter. I More funds and more resources are prepared to bridge the gap between us."

After a pause, Yang Rui said again: "G-protein coupled receptor is a world-class project.

After all, it is necessary to compete with foreign first-class laboratories, so everyone, including me, must grow up as soon as possible. "

As soon as he said that, Su Xiankai's expression eased.

However, the pressure of reality quickly fell on Su Xiankai.

"Just adding two people and a batch of machines can't make up the gap between us and foreign first-class laboratories." Su Xiankai noticed the loophole in Yang Rui's logic.

Yang Rui curled his lips and said, "If we do projects like top-notch foreign labs, it will definitely not work, but our goal is to concentrate materials on bovine rhodopsin protein."

Several people in the laboratory looked at Yang Rui for no reason.

Su Xiankai even asked: "Isn't our material just bovine rhodopsin protein?"

"We are, but that's not necessarily the case for foreign laboratories."

"why?"

"Because they want better materials and want to try more templates, this is our opportunity." Yang Rui still didn't say a word, that is, he knew that at this stage, bovine rhodopsin protein is the best of the material.

As a protein that can transmembrane seven times, the g-protein coupled receptor has a very complex molecular structure, and this complex molecular structure, even if it is the reason why human beings study it, is also the reason why it hinders human research.

Bovine rhodopsin is the most stable and clearest seven-transmembrane protein that scientists have found.

At least at this stage, it does.

Of course, the ambitions of scientists are unlimited, and they will always want better materials.

Yang Rui also wants, when his laboratory becomes the world's top laboratory, even if it costs twice or three times the funds, he will still explore such a world.

In China in 1986, the Ion Channel Laboratory of Peking University was not qualified to do so.

In ordinary laboratories, it is common practice to select one material, or to select a variety of experimental materials in order to disperse risks, and to conduct experiments directly.

Yang Rui put his confidence in the bovine rhodopsin protein, that's all.

This kind of knowledge is taken for granted by Yang Rui, but it is a little uncommon for Su Xiankai and others. Thirty years later, even if domestic graduate students do not read relevant papers, they can still learn a lot of relevant information from their classmates and teachers, but in China in 1986, there was no such condition.

Ordinary domestic scholars have no idea of ​​how top-notch foreign laboratories conduct experiments and how top foreign laboratories conduct scientific research.

Not to mention the management of the laboratory, it is all a secret that does not need to be kept secret.

However, Yang Rui knew very well that in the field of G-protein coupled receptors, everything was chaotic. It was the same in 1986 and 2016.

It's just that Yang Rui can see a little more information than other people.

For example, finding the most appropriate material in a chaotic field of vision.

"I asked someone to send a batch of bull's eyes over." Yang Rui stood up, made a phone call, said a few polite words, then hung up and said, "It's done, Lao Su, let's double the size, no problem. "

"Double?" Su Xiankai said somewhat unaccustomed: "Shall we do it step by step, and increase it by about 20% first?"

Yang Rui sighed, and said, "Double the increase, it's a step by step."

He glanced at Gu Qiang and Fan Zhenlong, and said: "Don't listen to them saying that it is twice as much as expected, it is true. Judging from your current progress, I think it is far from enough for you to provide twice as much bovine rhodopsin protein." Not enough."

Gu Qiang was not happy anymore, and said: "Director Yang, you look down on people."

Yang Rui raised his eyebrows and asked, "Tell me something to make me respect, what step have you guys made now?"

Gu Qiang groaned for a long time and said, "We are getting close."

"If it weren't for watching you guys make dim sum, do you think I would add more materials to you?" Yang Rui gave a half-sentence and half-encouragement sentence, saying: "Don't be naughty, you have to learn a lesson, don't let the old Su's hard work was in vain."

Su Xiankai's face had long since collapsed, and he said, "I feel like I'm a coolie. Director, I'm also the deputy team leader. Can't we give me a more dynamic job?"

"Your work is energetic enough. In addition, our main work recently is to produce bovine rhodopsin protein, so your side is the main direction of attack..."

"You lied to me." Su Xiankai was full of doubts.

Yang Rui said solemnly: "In the next month or two, the place where there will be a large increase in funds, materials, equipment and personnel will be the production of bovine rhodopsin protein, understand?"

Su Xiankai believed it all at once, and just frowned and asked, "Why?"

"Why? Because your teammates have a low level of experimentation, are slow in doing things, consume a lot of energy, and have no other good ideas, so you can only feed them with materials."

Yang Rui's aggressive attacks one after another finally excited Gu Qiang.

Comrade Gu Qiang jumped at the table and said: "Look carefully, we don't need to feed, and I will increase the success rate in the next day."

"That's right, I can speak Beijing dialect, and Erhuayin can pronounce it decently." Yang Rui suddenly put on a smile on his face, which caught people off guard.

Gu Qiang was so choked that he had no choice but to continue talking on the topic of Erhuayin.

Xu Zhengping coughed at this moment and asked, "Yang Rui, do you really want to double the production of bovine rhodopsin protein?"

"Yes, this is our only chance to catch up with foreign laboratories." Yang Rui deliberately said something serious, and then said: "We must realize the strength of the enemy, and we must not underestimate ourselves. The success rate of your experiments is indeed very high. High, but what about the researchers in foreign laboratories? How many times can they succeed? It is also a matter of uncertainty. This is a big project, and there are still many experiments to follow, which will be very long. We must gain advantages from the source, so as to eliminate The other forces of the enemy, right?"

"Don't foreign laboratories want to increase the success rate from the source?" Fan Zhenlong asked.

"First, foreign laboratories cannot be sure that the most appropriate material is bovine rhodopsin protein. Second, even if they are aware of it, they still hope for the success rate of the next experiment. Third, they obtain fresh bovine rhodopsin protein. Even the most eye-catching channels can’t keep up with us.”

The last one is Yang Rui's half-truth and half-hypothesis.

To some extent, after Yang Rui established the genetic engineering laboratory, his relationship with various animal husbandry companies can provide him with a large amount of animal materials, limited to such a small point, which is the world's top condition.

When Fan Zhenlong heard Yang Rui's second point, he was a little stunned. He knew Yang Rui's speaking habits too well.

Fan Zhenlong couldn't help asking suspiciously: "What do you mean they put their hopes on the success rate of the next experiment?"

"It's literally." Yang Rui coughed.

"No, when you say hope, you mean that there should be no hope."

Yang Rui laughed twice, but didn't answer directly.

Fan Zhenlong asked again and again.

Yang Rui considered his words and said, "Let's put it this way, it's more accidental."

Not just by chance, the three-dimensional structure of g protein-coupled receptors, like various space exploration plans, is completely piled up with numbers.

What quantity to stack out?

Bovine Rhodopsin!

At this point, the top foreign laboratories have already ignited and lagged behind.

Only when they realized that bovine rhodopsin protein was the best material, and only when they realized that a lot of material was needed to stack the 3D structure of the g-protein coupled receptor, did they catch up with the ion channel lab .

Of course, once top foreign laboratories realize this, they will catch up extremely quickly.

Yang Rui could only run as fast as possible.

Fan Zhenlong was completely shocked by Yang Rui's answer.

He hesitated for a long time and asked, "How coincidental?"

"Don't worry, I will organize the production of enough materials." Yang Rui patted Fan Zhenlong on the shoulder to make him feel relieved.

Fan Zhenlong's heart is about to explode!